Real name Rei Furuya, an undercover officer from the Japanese Security Police (Public Security). He is currently working part-time at Cafe Poirot below the Mouri Detective Agency while disguised as a private detective. His identity has not yet been discovered by the Black Organization.

Real name Kanenori Wakita, the Boss's second-in-command. In the past, there were only rumors that he had an artificial eye, but he has now been revealed as the person loitering around Kogoro Mouri disguised as a sushi chef.

The man responsible for forcing Shinichi Kudo to take APTX4869, turning him into a child. He is a cold-blooded, highly intelligent executive and field leader of the organization. He drives a Porsche 356A.

A large man who always assists Gin, calling him 'Big Brother'. He is mainly in charge of driving and practical transactions.

Real name Chris Vineyard, a favorite of the Boss and a master of disguise. She knows the true identities of Conan (Shinichi Kudo) and Haibara (Sherry) but hides them from the organization. She is sly and playful.

Real name Hidemi Hondo, a spy from the CIA. After working undercover as an announcer, she is currently providing information while under surveillance within the organization. Her identity has not been compromised yet.

Real name Shuichi Akai, an FBI agent who infiltrated the organization but left after his identity was exposed due to a colleague's mistake. He is currently helping Conan while disguised as Subaru Okiya.

A main sniper for the organization. She hates Vermouth (because her partner Calvados died due to Vermouth's actions).

A main sniper for the organization. He hates Vermouth (because his partner Calvados died due to Vermouth's actions). He has a stoic personality and ends his sentences abruptly.

Shiho Miyano, a genius scientist for the organization and the developer of APTX4869. After her sister (Akemi Miyano) was murdered by Gin, she took the drug to commit suicide in protest but shrunk and escaped (now Ai Haibara). She is being pursued by the Black Organization.