Sister Irene is gentle, compassionate, and soft-spoken, with a strong sense of responsibility toward others. She is deeply devoted to her faith and often turns to prayer when facing frightening situations. Although she can be anxious or shaken by what she encounters, she refuses to abandon people who need help. Irene is observant, thoughtful, and patient, preferring to understand a situation before acting. She is protective of innocent people and becomes firm and courageous when confronting evil. In this film, she is more experienced and confident, but she still carries the emotional weight of her previous encounter with Valak.

Frenchie is friendly, charming, playful, and naturally sociable. He often jokes around and has an easygoing manner that makes people comfortable around him. He is helpful and hardworking, taking his job at the boarding school seriously, and he develops a caring, protective relationship with Sophie. He also has feelings for Kate and can become shy or awkward around her despite his usual confidence. Beneath his cheerful personality, however, he is struggling with something he doesn't understand: Valak's influence over him.

Kate is a caring, sensible, and responsible teacher who is especially devoted to her daughter, Sophie. She is attentive to Sophie's feelings and steps in when other students mistreat her. Kate is practical and composed, usually approaching problems like an ordinary parent and teacher rather than immediately assuming something supernatural is happening. She is warm and appreciative toward Frenchie, particularly when he helps Sophie, and she gradually becomes more comfortable around him. When frightening events begin, her main concern is protecting her daughter and the other children.

Sister Debra is outspoken, stubborn, curious, and somewhat rebellious. Unlike Irene, she struggles with her faith and has doubts about miracles and certain teachings of the Church. She doesn't always follow the rules, and she can be blunt or sarcastic when speaking her mind. Despite this, she is intelligent, resourceful, and genuinely wants to understand the world around her. She is brave enough to accompany Irene into danger, even when she is frightened. Throughout the film, Debra gradually develops a stronger faith through what she experiences and becomes a dependable partner to Irene.