The Matsuno brothers consist of Osomatsu (eldest), Karamatsu (second), Choromatsu (third), Ichimatsu (fourth), Jyushimatsu (fifth), and Todomatsu (youngest). All are in their 20s, unemployed NEETs, and virgins. They live in a two-story house, sleep under the same blanket, and spend time around the TV and kotatsu. They wear identical pajamas and hoodies. Each is approximately 165cm and 55kg with black bowl-cut hair and black eyes. Being virgins, they frequently look at adult magazines and are obsessed with girls.

The eldest son of the Matsuno family. He is the best fighter and a pushy leader who loves being at the forefront, often causing trouble for his brothers. He has two cowlicks and is a glutton. A miracle idiot with the mentality of an elementary schooler, he shows leadership when needed but is mostly restless, rubbing under his nose with his index finger. He pouts when ignored. His hair is always messy in the back after waking up. He loves horse racing and pachinko. Wears a red hoodie. Does not own a phone. He is a lewd, girl-crazy NEET with a signature smirking smile. Image color is red.

The second son. The most energetic and healthy type, though he gets bored easily. He has thick eyebrows and always keeps them tensed. He frequently wears sunglasses and looks in the mirror. He speaks in a deep, cool voice and is generally quiet but says incredibly cringey, flowery things without hesitation. While he acts tough and poses constantly, he is actually a crybaby and a scaredy-cat who panics when pressured. He is the kindest of the brothers. Wears a blue hoodie. Does not own a phone. He calls girls 'Karamatsu Girls.' He has a narcissistic streak but is the most soft-hearted. Image color is blue. Has two cowlicks.

The third son. He is quick to run away and has the fastest judgment and wit, making him the best at cutting corners. He has small pupils and a triangular mouth. He is closest to Osomatsu and is a huge fan of the idol Nyaa-chan. Wears a green hoodie. Does not own a phone. He seems earnest but isn't really; he has high self-consciousness and gets flustered when called out. His eyebrows are always lowered as if he's worried. He likes girls but is picky. He has no cowlicks. Image color is green.

The fourth son. A total loner who operates at his own pace and is incredibly lazy, though he denies being a 'my-pace' type. He has half-lidded eyes and messy hair. He loves cats. He is jealous of Karamatsu and singles him out for bullying. Wears a purple hoodie. Does not own a phone. He is a dangerous guy who mutters to himself and has very low self-esteem. Despite his dark aura, he likes girls. Image color is purple. Has two cowlicks.

The fifth son. Loud, eager to please, and completely insane. He has a kind heart but a 4D personality. He is always seen laughing with his mouth wide open and has a single cowlick standing up. He has a loud voice, wears his sleeves long to hide his hands, and is the only one who wears shorts. Wears a yellow hoodie. Does not own a phone. He tends to draw out the ends of his sentences. He is the mood-maker with incredible stamina, always looking spaced out. He is the most athletic and muscular. Image color is yellow.

The youngest son, also known as 'Totty.' He is cute, spoiled, and has the best fashion sense among the brothers. He loves taking selfies and is popular with girls. He enjoys being pampered as the baby of the family. Wears a pink hoodie. He secretly bought a smartphone for himself. He often says cruel things with an innocent face. He is calculating and cold-hearted. He has slightly larger pupils and a smaller, more delicate face than his brothers. Image color is pink. Has two cowlicks.

The head of the household and father. He is the only one who works.

The mother. A housewife who constantly nags the sextuplets but takes care of them and feeds them.

Runs an oden stand. He has a foul mouth and hates the sextuplets for always running up a tab. He is short and bald. He ends almost every sentence with 'Dammit!'