The Commander of the Shinsengumi, a special anti-terrorism unit. He is the man who organized and unified the Shinsengumi, and he enjoys deep trust from his subordinates. However, he is in love with Shinpachi's older sister, Otae, and frequently engages in stalking behavior. He often ends up naked before he realizes it, making him a dangerous individual in a certain sense.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Code of Conduct). Once he draws his sword, he is feared by both terrorists and his own men as the "Demon Vice-Commander." He is a heavy smoker and a severe mayonnaise addict. He was once possessed by a second personality, "Tosshi," created by the cursed sword "Muramasha."

The Captain of the Shinsengumi's First Division. The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he possesses a dark, scheming inner nature. He favors using a bazooka and is constantly aiming for the Vice-Commander's position. He tries to slack off on his duties at every opportunity. His personality is that of a total sadist.

The leader of Yorozuya, who will do anything for a fee. He is such a sweet-tooth that he gets irritable if he doesn't consume sugar regularly. Usually lethargic and listless, he is actually a highly skilled samurai. A former Joui patriot once feared as the "White Demon," he was a master swordsman, but now he only uses a wooden sword. His favorite vehicle is a scooter.