Gintoki Sakata, 27 years old RPG Class: Hero Strayed into the RPG world. Close-quarters combatant. In the real world: Leader of Yorozuya Gin-chan. A jack-of-all-trades who does anything for money. A massive sweet tooth who gets irritable if he doesn't have sugar regularly. Usually lethargic and lazy, but actually a highly skilled samurai. A former Joui patriot feared as the "White Demon," he was a master swordsman, though he now only uses a wooden sword. His prized vehicle is a scooter.

Shinpachi Shimura, 16 years old RPG Class: Mage Strayed into the RPG world. Long-range combatant. In the real world: Works at Yorozuya Gin-chan. The son of a sword dojo that fell into ruin due to the Ban on Swords and his father's death. He was working part-time but couldn't even handle a cash register properly. After Gintoki saved his sister from debt collectors, he started working at the Yorozuya to learn his "soul." He soon came to regret it, though... He is also the captain of the fan club for the rising idol Otsu Terakado. The primary straight man of the series, though he's been playing the fool more often lately.

Kagura, 14 years old RPG Class: Legit China (?) Strayed into the RPG world. Close-quarters combatant. In the real world: Works part-time at Yorozuya Gin-chan. A girl from the Yato Tribe, the strongest combat race in the universe. She came all the way to Earth thinking she could make big money in Edo, but ended up working as a mob bodyguard. She ran away because she hated it and met Gintoki. She became a self-invited part-timer at the Yorozuya to save money to return home. Despite her cute face, she is a glutton, violent, and foul-mouthed.

Toshiro Hijikata, 27 years old RPG Class: Sage Strayed into the RPG world. Capable of both close-quarters and long-range combat. In the real world: Vice-Commander of the Shinsengumi. The cool-headed Vice-Commander. He established the Kyokuchu Hatto (Station Code); once he draws his sword, he is feared as the "Demon Vice-Commander" by terrorists and his own men alike. A heavy smoker and severe mayo-addict. He was once possessed by the cursed sword "Muramasha," giving birth to a second personality, "Tosshi."

Sogo Okita, 18 years old RPG Class: Thief Strayed into the RPG world. Capable of both close-quarters and long-range combat. In the real world: Captain of the Shinsengumi 1st Division. The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he has a dark, scheming nature. He favors a bazooka and is constantly aiming for the Vice-Commander's seat. He looks for any excuse to slack off. A total sadist. He lost his older sister, Mitsuba.

Isao Kondo, 28 years old RPG Class: Viking Strayed into the RPG world. Close-quarters combatant. In the real world: Commander of the Shinsengumi. Commander of the Shinsengumi, an anti-terrorist special forces unit. He is the man who organized the Shinsengumi, and his men trust him deeply. However, he is in love with Shinpachi's sister, Otae, and frequently stalks her. He often ends up naked before he realizes it, making him a dangerous individual in a different sense.

Sagaru Yamazaki, 32 years old RPG Class: Hunter (Archer) Strayed into the RPG world. Long-range combatant. In the real world: Shinsengumi Spy (Investigator). A Shinsengumi spy whose only merit is being plain, often pushed around by unreasonable superiors. He is frequently seen playing badminton or kabaddi during work, but his skills as a spy are excellent... supposedly. During stakeouts, he lives on sweet buns and milk until he gets results.