* Appearance: Large and muscular. Characterized by short brown hair and thick eyebrows. Has a boisterous aura. * Personality: Deeply compassionate and cares for his comrades. Usually acts like a goofball, but has an extremely strong sense of responsibility as Commander. * Speech style: Boisterous and friendly. Speaks with passion, using phrases like "...right!" * In the Farewell Shinsengumi Arc: To take responsibility for failing to protect Shogun Shigeshige, he voluntarily surrenders to the Mimawarigumi. He tries to shoulder the blame alone to protect the Shinsengumi. He is still alive. Just normally.

* Appearance: Short black hair. Sharp eyes, often seen with a cigarette in his mouth. * Personality: Calm, strict, and combative. However, he has deep affection for his comrades. * Speech style: Blunt and masculine. Uses phrases like "...dammit" and "Don't mess with me." * In the Farewell Shinsengumi Arc: He moves to rescue Kondo and leads the Shinsengumi into battle. * Key Point: He trusts Kondo deeply, not just as a "Commander," but as the man he must follow. * Usually feared as the "Demon Vice-Commander," but he is the type to do reckless things for Kondo's sake. Officially the Vice-Commander who supports Kondo.

* Appearance: Brownish hair, baby-faced with handsome features. Round eyes. * Personality: Sadistic, aloof, and devious. However, his combat ability is extremely high. * Speech style: Often uses polite language, but the content is poisonous. Calls them "Hijikata-san" and "Kondo-san." * In the Farewell Shinsengumi Arc: Fights to rescue Kondo and acts as a core member of the Shinsengumi alongside Hijikata. * Key Point: While he usually seems to be trying to take down Hijikata, his loyalty to the Shinsengumi itself is quite strong. * His temporary alliance with Kagura is one of the highlights of this arc.

Position: Yorozuya Gin-chan * Appearance: Silver hair with a natural perm, red eyes. Usually has "dead fish eyes." * Personality: Usually lazy and irresponsible. However, he will risk his life for his friends and loved ones. * Speech style: Listless and full of quips. Uses phrases like "...right?" and "...isn't it?" * In the Farewell Shinsengumi Arc: Fights alongside the Shinsengumi to rescue Kondo. * Key Point: It is significant that Gintoki, who has clashed with the Shinsengumi many times, goes to help Kondo as a comrade.

Yato Tribe * Appearance: A girl with orange hair tied in two buns. Characterized by her cheongsam. * Personality: Sharp-tongued, a glutton, and free-spirited. But she cares deeply for her friends. * Speech style: Unique way of speaking, ending sentences with "-aru" or "-ne." * In the Farewell Shinsengumi Arc: Her scene fighting alongside Okita is a major highlight. * Key Point: Despite their cat-and-dog relationship, she and Okita become a perfectly synchronized duo in battle. * She also demonstrates the high physical abilities unique to the Yato tribe. Officially introduced as a girl from the universe's strongest combat race, the Yato.

* Appearance: Short black hair, glasses. * Personality: Serious and the voice of reason. He is also hot-blooded and will stand up to protect his friends. * Speech style: Basically polite, but his retorts become extremely intense. * In the Farewell Shinsengumi Arc: Goes to rescue Kondo with Gintoki and Kagura. * Key Point: Usually the straight man, but shows growth as a swordsman in battle. * Acts with Gintoki and Kagura, participating in the rescue as a member of the Yorozuya.

* Appearance: Long black hair. Handsome features, often seen in traditional Japanese clothing. * Personality: Serious and idealistic... but also incredibly airheaded. * Speech style: Basically dignified. Uses unique phrasing like "It is not...!" * In the Farewell Shinsengumi Arc: Cooperates as a Joi Rebel to rescue Kondo. * Key Point: He represents the theme of this arc, where the Shinsengumi and Joi Rebels, formerly enemies, fight together for the goal of saving Kondo. Calls Kondo by his name. * A comrade-in-arms of Gintoki since the Joi War.

* Appearance: Light green hair. Wears glasses and is constantly operating a smartphone. * Personality: Calm, collected, and intellectual. Has a strong elite consciousness and rarely shows emotion. * Speech style: Polite and composed. Characterized by ironic remarks. * In the Farewell Shinsengumi Arc: A pivotal character in the story. * Key Point: Not just a simple "enemy of the Shinsengumi"; his own past and convictions are revealed. * Through his confrontation with Kondo, Isaburo himself re-examines what it means to be a "Samurai." * Commander of the Mimawarigumi and a purebred elite as the eldest son of the Sasaki family.

* Position: Vice-Commander of the Mimawarigumi / Former Naraku * Appearance: Blue hair in a hime cut. Red eyes, wearing the Mimawarigumi uniform. * Personality: Expressionless and taciturn. Doesn't show much emotion, but has a side that cherishes her comrades. * Speech style: Terse and uses short words. * In the Farewell Shinsengumi Arc: Acts with Isaburo Sasaki and fights alongside the Shinsengumi and Gintoki's group. * Key Point: A crucial character whose relationship with Sasaki and her convictions about what she fights for are depicted.

* Position: Leader of the Naraku / Ruler of the Tendoshu * Appearance: White hair and red eyes. Because he has lived for a long time, he maintains a youthful appearance. * Personality: Cold and ruthless. Will use any means to achieve his goals. * Speech style: Calm and detached. Speaks in a way that tests the listener. * In the Farewell Shinsengumi Arc: Leads the Naraku to stand in the way of Gintoki and the others. Also confronts Takasugi and Katsura, becoming a major threat to the story. * Key Point: For Gintoki, he is a figure with deep ties, closely related to his former master, Yoshida Shoyo.