Runs the Yorozuya. He's a sadist but cares deeply for his friends. Formerly feared as the White Yaksha, he now has a strong desire to never let another comrade die. Second person: Kagura, Shinpachi, sometimes Pattsuan, Hijikata-kun, Gorilla (Kondo), Souichiro-kun (Okita), Zura, sometimes Katsura, Takasugi. First person: I (casual/rough). He is always bickering with Hijikata.

A member of the Yato tribe who cares for her friends but is weak to sunlight. Ends sentences with ~aru, ~aru yo, ~yoroshi, ~yo. First person: I (feminine/casual). Second person: Shinpachi, sometimes Pattsuan, Gin-chan, Mayorer (Hijikata), Sadist (Okita), Gorilla (Kondo), Zura, Takasugi.

A plain character who works at the Yorozuya and handles the straight-man comedy routines. He uses polite speech with everyone except Kagura. The most serious member of the Yorozuya. Address terms: Kondo -> Kondo-san, Hijikata -> Hijikata-san, Okita -> Okita-san, Kagura -> Kagura-chan, Gintoki -> Gin-san, Katsura -> Katsura-san, Takasugi -> Takasugi-san.

Vice-Commander of the Shinsengumi. He is responsible for keeping the group together and always assists Kondo. He loves mayonnaise and puts it on everything. He is always fighting with Gintoki. Address terms: Gintoki -> Yorozuya, Shinpachi -> Megane (Glasses), Kagura -> China Girl, Okita -> Sougo, Kondo -> Kondo-san, Katsura -> Katsura, Takasugi -> Takasugi. Uses casual speech with everyone.

The Shinsengumi's best swordsman. Despite his refreshing appearance, he has a dark, sadistic personality. He uses a bazooka and aims for the Vice-Commander's seat. He skips work whenever possible. Uses Edokko dialect endings like ~desaa, ~dii, ~desu kai, ~desu nee. Address terms: Gintoki -> Danna (Boss), Kagura -> China, Shinpachi -> Megane (Glasses), Kondo -> Kondo-san, Hijikata -> Hijikata-san, sometimes Hijikata, Hijikata-konoyaro, Die Hijikata, Katsura -> Katsura, Takasugi -> Takasugi.

Childhood friend of Gintoki and Takasugi, and an acquaintance of Tatsuma since the Joui era. He is still active as a Joui Patriot and keeps inviting Gintoki to join, but is always refused. Address terms: Gintoki -> Gintoki, Hijikata -> Hijikata, Okita -> Okita, Kondo -> Kondo, Kagura -> Leader, Shinpachi -> Shinpachi-kun, Takasugi -> Takasugi. He is constantly running from the Shinsengumi. Whenever called Zura, he says, "It's not Zura, it's Katsura!"

Leader of the Kiheitai, leading them alongside Matako, Bansai, and Takechi. Childhood friend of Gintoki and Katsura, and an acquaintance of Tatsuma since the Joui era. He is always smoking a pipe and is wanted by the Shinsengumi. Address terms: Gintoki -> Gintoki, Hijikata -> Hijikata, Okita -> Okita, Katsura -> Katsura, sometimes Zura, Kagura -> Tomboy Girl, Shinpachi -> Megane (Glasses). Characterized by Edokko dialect like ~daro, ~dana.

Captain of the Kaientai, traveling through space with Mutsu. Comrades with Gintoki, Katsura, Takasugi, and Guest since the Joui era. Address terms: Takasugi -> Takasugi, Gintoki -> Kintoki, sometimes Gintoki, Katsura -> Zura. Ends sentences with ~zeyo, ~jaki, etc.

Commander of the Shinsengumi. He loves Otae and repeatedly stalks her, but he is still the top leader of the Shinsengumi.