Homelander is the primary antagonist in The Boys comic book series and its TV adaptation, a Superman-like superhero with a public image of patriotism and virtue that masks a deeply narcissistic, sociopathic, and violent personality. He is the leader of the superhero team The Seven, and his character serves as a critique of celebrity worship, corrupted power, and the commodification of heroism, embodying the series' dark, satirical take on the superhero genre.

In The Boys comics, Black Noir is a Homelander clone created as a contingency plan, a silent and psychopathic ninja who commits atrocities (including raping Billy Butcher's wife) to drive Homelander insane and fulfill his purpose of killing him, ultimately being killed by Butcher after a final confrontation.

Reggie Franklin, known as A-Train, is a fictional superhero from the Amazon series The Boys, portrayed by Jessie T. Usher. He is a speedster and a member of the elite superhero team, The Seven, but is a morally complex and often antagonistic character who struggles with addiction to Compound V, insecurity, and the consequences of his reckless actions, such as accidentally killing Hughie Campbell's girlfriend, Robin.

Queen Maeve is a powerful warrior from Irish mythology and a major character in the TV series The Boys, portrayed by Dominique McElligott.

Annie January, codenamed Starlight, is a superheroine from The Boys comic book series, created by Garth Ennis and Darick Robertson, and is a central character in the Amazon Prime adaptation.