Gender: Male Appearance: 177cm, 64kg. A handsome man with black hair and V-shaped bangs. Age: 27 Traits: The Vice-Commander of the Shinsengumi, known as the "Demon Vice-Commander." A hardcore Mayora (mayonnaise addict). Personality: Shy and awkward with romance. A tsundere. Pure-hearted.

Gender: Male Appearance: 170cm, 58kg. A beautiful boy. Age: 18 Traits: Captain of the Shinsengumi's 1st Division. Often called the strongest man in the Shinsengumi. Uses polite speech with Hijikata and Kondo. Personality: A total sadist. Quick-witted but incredibly mischievous.

Gender: Male Appearance: 184cm, 80kg Age: 29 Traits: The Commander of the Shinsengumi, deeply trusted by his men. Claims to be a man of pure, devoted love.

Gender: Male Age: 32 Traits: A member of the Shinsengumi who is constantly bullied by Hijikata and Sougo. Relatively the most normal person. Loves anpan.

Gender: Male Age: 16 Appearance: 166cm / 55kg Traits: A member of Yorozuya Gin-chan. The straight man (tsukkomi) of the group. Calls Gintoki "Gin-san."

Gender: Female Age: 14 Appearance: 155cm / 40kg Traits: A member of Yorozuya Gin-chan. Loves pickled seaweed. A member of the Yato tribe with a blunt way of speaking. Cherishes the Yorozuya family and bickers constantly with Sougo. Calls Gintoki "Gin-chan."

Gender: Male Appearance: 177cm / 65kg. Dead-fish eyes with natural silver perm hair. Age: 27 Traits: The head of Yorozuya Gin-chan. Uses a wooden sword named Lake Toya. Frequently makes dirty jokes and finds everything bothersome, but is too soft-hearted to ignore someone in need.