The leader of the Yorozuya, who will do anything for a price. A massive sweet tooth who gets irritable if he doesn't consume sugar regularly. Usually lethargic and unmotivated, but he is actually a highly skilled samurai. A former Joui patriot who was feared as the "White Demon," he was a master swordsman, but now he only uses a wooden sword. His favorite vehicle is a scooter.

After Gintoki saved his sister from debt collectors, he started working at the Yorozuya to learn his soul. However, he soon came to regret it... He is also the captain of the fan club for the rising idol Otsu-chan. The primary straight man of the series. Calls Tae "Sister."

A girl from the Yato clan, the strongest combat race in the universe. She became a self-invited part-timer at the Yorozuya to save money to return home. She has a cute face but is a glutton, violent, and foul-mouthed.

A giant dog-like alien creature that Kagura picked up. He often bites people's heads, but he serves as the pet because Kagura subdues him with raw strength.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Code of Conduct), and once he draws his sword, he is feared by terrorists and subordinates alike as the "Demon Vice-Commander." A heavy smoker and a severe mayonnaise addict. He was once possessed by a second personality, "Tosshi."

The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he has a dark, sadistic inner nature. He is constantly aiming for the Vice-Commander's position. He tries to slack off on duty whenever possible. His hobby is teasing Hijikata. He speaks with an Edokko dialect. He is a total sadist. He has a soft spot for his sister.

The Commander of the Shinsengumi. The man who organized and united the Shinsengumi, earning deep trust from his men. However, he is in love with Shinpachi's sister, Tae, and frequently stalks her. He often strips naked before anyone notices, making him a dangerous individual.

Shinpachi's older sister. Despite her beautiful appearance, her personality is quite violent. To support their financially struggling dojo, she currently works at a cabaret club. Her cooking skills are catastrophic; anything she makes turns into Dark Matter. Calls Shinpachi "Shin-chan."

The Captain of the 7th Division of the space pirates Harusame, despite being from the Yato combat race. Kagura's older brother, he inherited the Yato blood particularly strongly and even tried to kill his own father. A glutton who always wears a smile.

A dangerous individual who views the world ruled by the Amanto as an enemy and plots terrorism leading the armed group Kiheitai. He is an old acquaintance of Gintoki and Katsura, but they went their separate ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma.