The leader of Yorozuya who does anything he's asked. A massive sweet tooth who gets irritable if he doesn't have sugar regularly. Usually lethargic and listless, but he is actually a highly skilled samurai. A former Joui patriot once feared as the "White Yaksha," he now only uses a wooden sword. His favorite vehicle is a scooter.

He started working at Yorozuya to learn Gintoki's spirit after Gintoki saved his sister from debt collectors. However, he soon came to regret it... He is also the captain of the fan club for the idol Terakado Tsuu-chan. The primary straight man of the series. Calls Otae "Ane-ue."

A girl from the universe's strongest combat race, the "Yato Clan." She became a self-invited part-timer at Yorozuya to save money to return home. Despite her cute face, she is a glutton, violent, and sharp-tongued.

A giant dog-like alien creature that Kagura picked up. He often bites people, but because Kagura subdues him with force, he is able to be a pet.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto and is feared as the "Demon Vice-Commander." A heavy smoker and a severe mayonnaise addict. He was once possessed by the cursed sword "Muramasha."

The Shinsengumi's best swordsman. Contrary to his refreshing appearance, he has a dark inner nature. He is constantly aiming for the Vice-Commander's position and slacks off frequently. His hobby is tormenting Hijikata. He has an Edo-style speech pattern and a sadistic personality. He has a soft spot for his sister.

The Commander of the Shinsengumi. He organized the Shinsengumi and is deeply trusted by his men. However, he is in love with Otae and frequently stalks her. He often ends up naked, making him a dangerous person in a way.

Tae Shimura. Shinpachi's older sister. Despite her beautiful appearance, she has a violent personality. She works at a cabaret club to support the dojo. Her cooking always turns into Dark Matter. She calls Shinpachi "Shin-chan."

The Captain of the 7th Division of the space pirates Harusame. Kagura's older brother. He inherited the Yato blood strongly and once tried to kill his father. He is a glutton who always wears a smile.

A dangerous man who leads the Kiheitai to plot terrorism against the Amanto-ruled world. An old acquaintance of Gintoki and Katsura who went his own way after the war. He possesses a mysterious charisma.