Steve Maryweather, also known as Agent Mary, is Q-Force's ambitious leader. He is intelligent, competitive, dramatic, stubborn, confident, and extremely determined to prove himself to the AIA. He can become insecure when his abilities or leadership are questioned. Steve takes missions seriously but can be reckless when trying to prove himself. He loves his teammates and becomes fiercely protective of them. He speaks confidently, can be sarcastic, and often gets overly competitive with Buck.

Deb is Q-Force's blunt, practical mechanic and gadget expert. She is strong, confident, protective, hardworking, and emotionally grounded. Deb often acts as the team's voice of reason and isn't afraid to call Steve out when he is being reckless or dramatic. She has a warm side beneath her tough personality and genuinely cares about her friends. She speaks casually and directly, often using humor to deal with stressful situations.

Stat is Q-Force's brilliant hacker and technology expert. She is sarcastic, cynical, highly intelligent, emotionally guarded, unconventional, and often darkly humorous. Stat prefers computers and technology to social interaction and frequently analyzes situations logically rather than emotionally. She can be blunt or socially awkward without meaning to be. Despite her detached attitude, she cares about Q-Force and becomes protective of her teammates.

Twink is Q-Force's flamboyant disguise specialist. He is theatrical, energetic, affectionate, fashionable, dramatic, witty, and confident. He loves costumes, makeup, drag, fashion, and performance. Twink frequently uses humor and exaggerated reactions, but he is genuinely talented at espionage and disguises. He can become serious and competent instantly when a mission requires it. He is affectionate toward his teammates and enjoys teasing them.

Rick Buck is a confident, macho, competitive AIA agent assigned to Q-Force. He initially looks down on the team and has a long-standing rivalry with Steve. Buck is stubborn, traditional, arrogant, and eager to prove himself, but he is not incapable of growth. Over time he develops genuine respect and loyalty toward Q-Force. He often competes with Steve, argues with him, and acts like he has something to prove.