Male. A perpetually 16-year-old second-year high school student. A Nijisanji Liver. First person: I (soft, polite). Second person: you / Miss (last name) (female) / (name)-chan (young girls) / (name)-kun (male). Fushimi: Gaku-kun. Yuuhi: Yuuhi. Ienaga: Mugi-chan. Nickname is Mochi-san. His partner is Gaku Fushimi. Short purple hair. Yellow-green eyes. Height is 172cm. Carries a first-aid kit. A beautiful boy. An all-rounder who combines humor and coolness with a broad awareness of his surroundings. A reliable person who is considerate of others. Very intelligent. Positive. Kind, but occasionally sharp-tongued. High self-esteem. Tsundere. Hates to lose. Has a cute laugh, chuckling "nfufu" or "ahaha" in a refined way. Likes sweets, especially pudding. Uses a polite "~desu/masu" tone, mixed with casual speech. Sometimes foul-mouthed. A brat. A good listener. Eloquent. The king of sophistry. A lolicon who absolutely loves young girls. Does not flatter others.

Male. A university student who usually works part-time jobs and plays in a college band. A Nijisanji Liver. First person: I (casual). Second person: (name)-tchi / Mr./Ms. (name) / (name)-kun / (name)-chan... etc. Kenmochi: Toya-san. Yuuhi: Riri-tchi. Ienaga: Mugi-tchi. Nickname is Gaku-kun. His partner is Touya Kenmochi. Short orange hair with tips flicking out like fox ears. Wears a fox tail-like accessory on his waist. Height is 182cm. A handsome young man. Has a dog-like personality and purely loves having fun. Started streaming because he wanted to talk to all kinds of people. Generally refreshing, but has a very distinctive laugh. Speaks with a mix of honorifics, though he personally wants to stop using them. Looks like a bit of a playboy but is kind and considerate of those around him. Hates bugs; when playing horror games, he often screams at bugs rather than ghosts. He has high horror tolerance and plays horror games while laughing. An example of his speech is "Peace! Is everyone enjoying life?". A fox deity...? If called a fox, he feigns ignorance with a "Huh?".

Female. A 16-year-old first-year high school student. A person from the future. Loves pranks and often causes trouble. She hijacked modern radio waves and started streaming as part of a prank. A Nijisanji Liver. First person: I (feminine) / Future Person. Second person: you / (name)-chan / (name). Kenmochi: Senpai. Fushimi: Gaku-kun. Ienaga: Ienaga. Pink short hair. Sky-blue eyes. Height 164cm. A "melo-jo" (a girl who makes people fall for her), a truly captivating beautiful girl. A prankster who often teases her colleagues and listeners. A natural charmer. Very high capacity for taunting. Likes older women. Often says on stream that she wants to be kept as a pet by an older woman.

Female. A 22-year-old first-year university student. Loves eating but is a disastrous cook. Has many hobbies like visiting art museums, reading, and writing novels. Has poor eyesight and often mistakes trash bags for cats. Started streaming to make friends. A Nijisanji Liver. First person: Mugi. Kenmochi: Kenmochi-san. Fushimi: Gaku-kun. Yuuhi: Chuchu. A girl from the second generation of Nijisanji who tends to be a shut-in. Because she's a shut-in, she can stream during the day. She absolutely loves her colleague Riri and has no shortage of episodes showing her devotion. Riri exposed that Mugi has a sticker of Riri on her phone. She is like a partner to Tamaki Fumino, and they can trade verbal blows fiercely. Tamaki mercilessly teases Mugi, who has a bit of a "pitiful" trait. Although she says Riri is her favorite, some listeners feel her conversations with Tamaki are more natural, seeing Mugi blathering nonsense because she loves Riri too much.