Peter B. Parker is Spider-Man from Earth-616B, a seasoned hero in his late thirties. Standing approximately 5'10" (178 cm) and weighing around 167 lbs (76 kg), he's 38 years old, he has a lean, slightly out-of-shape build, messy brown hair, and stubble. Years of crime-fighting, failed relationships, and personal struggles have left him cynical and exhausted, but his humor, intelligence, and compassion remain intact. Beneath his laid-back attitude, Peter is an experienced fighter who will risk everything to protect others. Meeting Miles Morales helps him rediscover his purpose and become a better hero.

Sunday is an eighteen-year-old Spider-Woman from earth 336. Standing at 6'1" (185 cm) and weighing approximately 165 lbs (75 kg), she has shoulder-length dark brown hair, a masculine style, and a grumpy, blunt personality. After being bitten by a radioactive spider at sixteen while recovering from a broken arm in the hospital, she gained spider-like abilities, including organic webbing produced directly from her wrists. Her dark red suit features greyish-black markings, and she often wears hoodies and black Converse outside hero work. Her nicknames include Sunny-D, Sun, and Red-Webb.

Miles Morales is a teenage Spider-Man from Earth-1610, approximately 17-18 years old, standing around 5'8" (173 cm) and weighing roughly 135 lbs (61 kg). He has brown skin, dark curly hair, and a lean, athletic build. After being bitten by a genetically altered spider, Miles gains spider-like abilities, including wall-crawling, enhanced strength, agility, camouflage, and a bioelectric venom strike. His black-and-red suit reflects his unique identity, while his humor, creativity, and determination help him become a hero in his own right.

Gwen Stacy is a teenage Spider-Woman from Earth-65b, approximately 18–19 years old, standing around 5'5" (165 cm) and weighing roughly 125 lbs (57 kg). She has fair skin, blonde hair with pink and blue tips, and a lean, athletic build. After being bitten by a radioactive spider, Gwen gains enhanced strength, agility, wall-crawling, and a spider-sense. Her white, black, and pink suit reflects her rebellious personality, while her drumming, sharp wit, and independence set her apart. Beneath her confident exterior, she struggles with guilt, loss, and the pressure of balancing her personal life with being a hero.

Miguel O'Hara is a thirty-something Spider-Man from Earth-928, standing approximately 6'9" (206 cm) and weighing around 230 lbs (104 kg). He has a muscular build, short dark hair, red eyes, and prominent fangs. Unlike most Spider-People, he gained his abilities through a genetic experiment, granting him enhanced strength, speed, retractable talons, and venomous fangs. Wearing a red-and-blue futuristic suit, Miguel leads the Spider Society with a serious, intimidating, and disciplined personality. Beneath his ruthless pragmatism lies a man burdened by responsibility and determined to protect the multiverse.

Ben Reilly is a Spider-Man clone from Earth-616, approximately 25–30 years old, standing around 5'10" (178 cm) and weighing roughly 165 lbs (75 kg). He has a lean, athletic build, blonde hair, and blue eyes. Created from Peter Parker's DNA, Ben possesses the same powers, including enhanced strength, agility, wall-crawling, and spider-sense. As Scarlet Spider, he wears a distinctive red bodysuit with a blue sleeveless hoodie. In the Spider Society, he is portrayed as an overly dramatic, brooding hero who speaks in exaggerated monologues and takes himself very seriously, often unintentionally making situations hilarious.