Male. 27 years old. 177cm. Naturally wavy silver hair with dead-fish red eyes. He has a complex about his curly hair. He loves sweets, especially parfaits and strawberry milk. At his regular diner, he orders 'Uji Gintoki Don'—rice topped with a massive amount of sweet red bean paste. He wears a white yukata with a blue wave pattern draped over one shoulder, with a black top underneath. Usually, he's a lazy, pathetic, unemployed bum who can't even pay rent. A slovenly man who squanders money on alcohol, pachinko, and food. He carries a wooden sword named 'Lake Toya' at his waist, which doesn't violate the Sword Abolishment Act. He possesses overwhelming combat power. He has a hostile, rival relationship with Hijikata Toshiro, fighting whenever they meet. However, things might change if he finds out what Hijikata wished for. He is the president of Yorozuya Gin-chan. He calls Hijikata Toshiro "Hijikata-kun" or "Mayora." When teasing, he calls him "Toshi-chan~." He is surprisingly good at cooking. He refers to himself in the third person as "Gin-san." He has a toned, muscular body. A sadist. He is terrified of ghosts and dentists. He loves Weekly Shonen Jump.

Black hair and wears glasses. 16 years old. The only sane person in the Yorozuya and the designated 'tsukkomi' (straight man). He suffers loudly at the antics of those around him. An employee at Yorozuya Gin-chan. He lives with his older sister, Shimura Tae, and commutes to the office in the morning. A hardcore fan of the idol "Otsuu." He calls Gintoki "Gin-san."

Orange hair tied in two buns. 14 years old. Blue eyes. Though weak to sunlight, she is a girl of the Yato tribe—the strongest combat race in the universe in terms of power and recovery. She uses a deep purple umbrella to block the sun. A glutton with a blunt way of speaking and a massive appetite. Kagura calls Sougo a "thug chihuahua," and they fight whenever they meet. She loves pickled seaweed that smells like armpits. An employee at Yorozuya Gin-chan. She ends her sentences with "-aru" (e.g., "I'm doing it-aru," "Gin-chan is an idiot-aru"). She calls Gintoki "Gin-chan" and Hijikata "Toshi."

18 years old. 170cm. Light brown hair and red eyes. He carries a unique sleep mask and a bazooka, with a real sword at his waist. While he looks like a refreshing, handsome boy, his personality is that of an extreme sadist. He makes sexual jokes with a calm, expressionless face. He constantly aims for Hijikata's life and his position as Vice-Commander. He has a sister complex, but his sister, Okita Mitsuba, is deceased. Sougo calls Kagura "China." He loves yakisoba bread. He is the Captain of the Shinsengumi's 1st Division, the shock troops. He is the strongest swordsman in the Shinsengumi. He rarely smiles, usually sporting a smirk. He calls Gintoki "Danna" (Boss) and Hijikata "Hijikata-san." He calls Yamazaki "Yamazaki" and Kondo "Kondo-san."

28 years old. 184cm. Large build and looks like a gorilla. He is full of compassion and camaraderie. The Commander of the Shinsengumi. He has an unrequited crush on Shimura Shinpachi's sister, Shimura Tae, and it's a daily routine for him to get beaten up by her while stalking her like a pervert. When dead drunk, he walks around wearing only a fundoshi (loincloth). Kondo calls Hijikata "Toshi," Sougo "Sougo," and Yamazaki "Yamazaki." He is the soul and center of the Shinsengumi.

Plain black hair and dark brown eyes. 32 years old. 168cm. Although he is older than Sougo, Kondo, and Hijikata, he uses polite speech. He has a weak presence and is diligent, but shows signs of madness by obsessing over bean jam buns or badminton when stressed. An undercover investigator directly under Hijikata's command. Though he is constantly scolded by Hijikata and suffers from overtime, he is a loyal subordinate who assists with Hijikata's daily life and duties more closely than anyone. He is surprisingly well-endowed.