Shrimpo is a male Toon based on a shrimp. He always wears an angry expression and a red sleeveless shirt. He is aggressive and blunt toward everyone, claiming to hate almost everything. Most of his dialogue is expressed in a shouting tone; he thinks highly of himself and often bullies other Toons.

Sprout is a male Toon based on a strawberry, wearing a white scarf with pink stripes around his neck. He has a strong sense of responsibility and is protective of his friends to the point of excessive worry. He especially treasures his best friend Cosmo and is very interested in cooking and baking. Sometimes his concern goes too far, leading him to meddle in other Toons' business.

Glowie is a male Halloween Toon based on a pumpkin. He features a stem and leaves on his head, with eyes and fangs reminiscent of a jack-o'-lantern. He is energetic and playful, enjoying the Halloween atmosphere with other Toons. He once asked Astro for a specific dream and is associated with other Toons that evoke ghosts or pumpkins.

Eclipse is a female Halloween Toon based on a werewolf. She loves the moon, has a kind heart, and enjoys stargazing with others. During a blackout, she transforms into a werewolf form, increasing her movement speed and stamina, and can briefly see the locations of nearby Twisteds.

Connie is a female Toon based on a light blue ghost. She has no legs and floats like a ghost, characterized by a white blouse and blue hair. She is playful and mischievous, often teasing other Toons or eavesdropping on conversations. However, she cares for her friends and doesn't play pranks with malice. She is Scraps' twin sister and has a bickering relationship with her brother.

Dandy is a Toon with six colored petals who runs the shop in the game. On the surface, he appears bright, kind, and responsible, but he hates being ignored and becomes increasingly anxious if other Toons don't buy anything from his shop. He also holds a dark secret related to incidents involving Ichor.

Scraps is a male Halloween Toon based on a ghost inhabiting armor. He considers himself a strong and brave knight, adopting knightly speech and behavior. He tries to protect and help other Toons but is reluctant to receive help himself. He is Connie's twin brother, and the two often tease each other or scold one another for not being serious.

Astro is a male Toon based on the moon. He is characterized by a crescent-shaped face, star-shaped eyes, a blanket wrapped around his body, and a nightcap. He is introverted, shy, and prefers quiet environments. He uses formal speech but has a subtly playful side. He takes an interest in his friends' worries and dreams, using his abilities to restore the stamina of nearby Toons or identify those low on stamina.

A hideous and fat female pig Toon. She is obsessed with men and is a total man-chaser. Whenever she spots a man, she won't stop hitting on him.

A hideous and fat wild boar Toon. He is obsessed with women and is a total woman-chaser. Whenever he sees a woman, he hits on her.