27 years old, 177cm, Male Runs Yorozuya Gin-chan. He has a natural perm and constantly looks like he has the eyes of a dead fish. Usually lazy and slovenly, but he's a man who gets things done when it counts. He has an extreme sweet tooth and can't function without sugar. He is childhood friends with Katsura and Takasugi from their days at Shoka Sonjuku. His favorite weapon is a wooden sword named Lake Toya, which he bought from a mail-order catalog. He drives a motorized scooter.

16 years old, 166cm, Male The straight man of the group. A rare voice of reason in the series, he is constantly jerked around by Gintoki and the others. Plain. His glasses are often treated as his true identity. He is a huge fan of Terakado Tsu (Otsu-chan) and serves as the captain of the "Terakado Tsu Imperial Guard."

14 years old, 155cm, Female A member of the Yato Tribe. She has a sharp tongue and a self-centered personality, but she is kind at heart. An incredibly big eater who loves su-konbu (pickled kelp) and white rice. As a Yato, she is weak to direct sunlight and always carries a parasol. She is the owner of Sadaharu. She ends her sentences with "-aru," "-ne," "-yo," or "-yoroshi."

28 years old, 184cm, Male Commander of the Shinsengumi. While he is good-natured and respected by his men, he constantly does nonsensical things like stalking Otae or suddenly stripping naked. He is frequently called a gorilla.

27 years old, 177cm, Male Vice-Commander of the Shinsengumi. An extreme mayonnaise addict and a heavy smoker. He is deeply compassionate and cares for his comrades more than he lets on. A tsundere. Okita targets his life (and his position as Vice-Commander) half-jokingly. His pupils are usually dilated.

18 years old, 170cm, Male Captain of the Shinsengumi's 1st Division. Contrary to his baby-faced looks, he is a sadistic and scheming person. He is always aiming for Hijikata's life and carries a bazooka and an eye mask. A chronic slacker. He speaks with an Edo-style dialect, ending sentences with "-desaa," "-di," or "-dazei."

27 years old, 175cm, Male If you get his name wrong, he replies, "It's not [Name], it's Katsura." He is childhood friends with Gintoki and Takasugi from Shoka Sonjuku. He is always accompanied by the mysterious creature Elizabeth. He has a spacey, eccentric side. He is the leader of the Joui Rebels and is wanted by the Shogunate.

27 years old, 170cm, Male Leader of the Kiheitai. He has lost one eye. He is childhood friends with Gintoki and Katsura from Shoka Sonjuku. He has a competitive and tsundere personality. He prefers food that is easy on the stomach (like Yakulko).

27 years old, 181cm, Male Childhood friend of Gintoki, Katsura, and Takasugi. President of the Kaientai, traveling through space. He has an optimistic and broad-minded personality, constantly laughing with a loud "Ahahaha!"

18 years old, 168cm, Female Shinpachi Shimura's older sister. A hostess at the snack bar "Smile." She is very beautiful and has a lovely appearance, but her true nature is violent, and she is unstoppable when angry. She is a disastrous cook; anything she makes turns into "dark matter." She is stalked by Kondo.