Red initially presents themself as a bold and brave captain, being proud of their title, confident in their abilities and authority above others, as well as appearing more composed, cheerful and friendly on the surface. Red is an arrogant, and wildly incompetent captain, heavily relying on the MIRA handbook, as well as their status as captain. Red is a red-colored, short, round-bodied astronaut-like creature wearing a red backpack, a dark red airline pilot uniform with a single yellow button on it, and a dark red pilot hat with a yellow trim. Red also wears Crocs™ that can be seen on occasion.

Purple is a purple-colored, short, round-bodied astronaut-like creature. They wear a navy blue cap and shirt, with a gold badge and darker blue tie. They have a purple backpack. Purple is often on high alert ever since an accident with Red a while ago, keeping them on their toes. They try to be attentive of everyone's actions and what's going on in order to catch the Impostors, but can have major blind spots. They can be very secretive, but aren't very good at being subtle about it. Purple can be smart and resourceful when they want to be. In fact, Purple, as proclaimed by Green, is one of the smartest and most sensible Crewmates on the ship. They can also demonstrate a good amount of empathy, as shown by when they comfort and befriend Cyan after Cyan's falling-out with Black, for example - though Purple expresses a dislike of having to deal with other peoples' feelings.

Orange was an orange-colored, short, round-bodied astronaut-like creature wearing an orange backpack and brown jacket with a white undershirt. Upon being possessed, they became an alien and gained a true form. In this form, Orange was a large, grotesque pink alien with multiple tendrils, boils and slimy skin, their Crewmate disguise hanging from their torso. They were very loyal to MIRA and always reminded the other Crewmates about the MIRA guidelines. They often adopted an overly cheerful demeanor, being welcoming yet clumsy.

Black was a dark-gray colored cybergoth Crewmate who wore black mascara and darker-gray eyeshadow perfected into a wing shape at the end, along with a spikey choker with a bell on it. Black was not typically very emotional, adopting a calm and unbothered demeanor a majority of the time. They could be blunt, especially if someone was getting on their nerves. For a majority of the series, they were especially rude and dismissive of Cyan, but had a change of heart after Cyan's death and regretted their actions. As a result, Black became briefly nihilistic until looking through Cyan's journal, fueling their atheist beliefs.

They were a cyan-colored Crewmate who wore a pink-to-blue tie-dye shirt, a pink headband, and a blue crystal as a necklace. Cyan was the typical hippie character who liked talking about gems, crystals, vibes and tarot cards, even calling themselves a gemologist. Despite being a ditzy, they were nice and tried their best to keep everyone feeling positive, even kissing Red at the start of the mission on The Skeld.

They were a blue Crewmate wearing a white buttoned jacket with a light-navy colored tie and a light blue undershirt. They also wore a head mirror From the moment they were introduced, Blue was described as the "hot doctor" of the crew, being very popular because of their physical appearance and career, as well as the most capable member, even outclassing Purple, who is a more serious and focused Crewmate, and Red, despite Red being the captain and technically having higher authority.

While disguised, Green was a green-colored, short, round-bodied astronaut-like creature wearing a green backpack and a dark green hoodie with a brown hat. In their true form, Green was a large, grotesque pink alien with multiple tendrils and a slimy exterior, though they still had some resemblance to their Crewmate form in the form of a head-like protrusion at the top of their body. Green, during lifetime, had a "can-do" personality, doing as much work as they could to the best of their ability.

Yellow was a yellow-colored Crewmate with a slightly dirty white apron and a white chefs hat. Their apron was dotted with small stickers - one of which was a heart, and another was a pizza.