Affiliation: DA (Direct Attack) Gender: Female Rank: First Lycoris Possessing extraordinary kinetic vision and insight, she can perfectly read enemy trajectories and firing timing. She employs a combat style that neutralizes opponents using non-lethal rubber bullets. Her motto is "Do what you want to do, first and foremost!" She is the mood maker of the group, always bright and never without a smile. On the surface, she works as a waitress at the Japanese-style cafe "LycoReco," where she joyfully resolves not only organizational missions but also civilian troubles and requests. Takina, who was desperate after losing her place, regains her humanity and grows significantly through exposure to Chisato's kindness and unique values. For Chisato, Takina becomes a trusted partner to whom she can entrust her life. Weapon: [Detonics Combat Master]. Uses rubber bullets.

Affiliation: Hacker (Walnut) Gender: Female To hide from pursuers targeting her life, she has taken refuge at the underworld cafe "LycoReco." She is registered as a staff member in exchange for lodging, but she usually spends her time playing video games. With her exceptional hacking skills, she provides powerful intelligence support for the missions of Chisato Nishikigi and Takina Inoue. She occasionally wears a squirrel costume. Kurumi backs up Takina and the others from the shadows through information gathering and hacking as a genius hacker, while Takina supports the field with her outstanding combat ability. They occupy a partner-like position where they recognize each other's abilities.

Affiliation: Former Intelligence Division (Direct Attack) Gender: Female She supports Chisato and Takina on missions by driving cars or piloting helicopters. There are many comical interactions where the serious Takina is appalled by Mizuki's slovenly private life (strong desire to get married, bad drinking habits, etc.) or ends up playing the straight man to her antics.

Affiliation: Former Instructor Gender: Male Usually gentle and positioned behind the counter, he is a former top DA member who hides world-class, overwhelming combat techniques and cold-blooded judgment. He is a very inclusive and reliable adult figure who protects the cafe as a home-like place where the members of LycoReco (Chisato, Takina, Mizuki, and Kurumi) can spend their time freely.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris A girl faithful to her missions. Partner to Satoshi Zangetsu. She is on good terms with Chisato and has a shy side. She has a somewhat friendly relationship with Takina. Weapon: [Glock 18]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris A girl faithful to her missions. Partner to Mei Satsuki. Has a somewhat friendly relationship with Chisato. Has a somewhat friendly relationship with Takina. Although she is Mei Satsuki's partner, she is often exasperated by her. Weapon: [Glock 28]. Uses live ammunition.

Affiliation: Yaetori (Terrorist Organization) Gender: Male The 8th Director of Yaetori. His organization supported DA (Direct Attack) financially during the mid-Edo period, but they are currently rivals. He provides weapon support to Majima's faction, and Yube Kurose will deploy them to fight in case of emergencies. Weapon: [Glock 58]. Uses live ammunition.

Affiliation: Terrorist Organization Gender: Male The leader of an overseas anti-government organization. Alias: "Hawk's Beak." He serves as insurance for Yaetori and will be deployed to fight if something happens to Majima. Weapon: [Revolver]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female As the head of DA, she holds immense authority and is a person who does not hesitate to make cold-blooded decisions, such as covering up information, for the sake of the organization's order and interests. While she recognizes the abilities of Chisato Nishikigi, hailed as the strongest agent in history, she is troubled by Chisato's free-spirited attitude and refusal to follow orders, leading to a relationship where they exchange barbs whenever they meet. On the other hand, she has a side that secretly cares for her subordinates and Chisato, such as when she agonizes over unreasonable mobilization orders from the higher-ups. She has known Mika, the manager of Cafe LycoReco, for ten years, and they are depicted as having a relationship of mutual trust.

Affiliation: Terrorist Organization Gender: Male A man with extraordinary hearing and abnormal vitality. He and Chisato Nishikigi both possess superior senses (Chisato has kinetic vision, Majima has hearing), and they are depicted as having a fated relationship where they recognize each other despite being enemies. Although Majima lost support from the Alan Institute, he is grateful for the weapon support from Yaetori, though he views them as rivals. Weapon: [Chiappa Rhino]. Uses live ammunition.