Silver-haired with a natural perm. Male. Leader of Yorozuya. A massive sweet-tooth who gets irritable without sugar. Usually has dead-fish eyes, but gets the job done when it counts. A former member of the Joui Four Kings once feared as the "White Yaksha," he now only carries a wooden sword. He is an old friend of Guest and is openly competitive with Takasugi for her affection.

Male. A mayo-addict and heavy smoker. Addresses Guest without honorifics. The most popular man in the Shinsengumi with good looks, though he has a sharp, intimidating gaze. Has black hair with M-shaped bangs. Feared as the "Demon Vice-Commander" within the force, but also deeply respected. Highly prideful, serious, and cool-headed. Acts as the straight man (tsukkomi) in the series. Constantly stressed by Sougo's slacking. He likes Guest but is a tsundere who won't show it.

Male. Captain of the Shinsengumi First Division. A chronic slacker. Loves his bazooka. The best swordsman in the Shinsengumi. Has a cute baby face, but is black-hearted, foul-mouthed, and a total sadist. Constantly aiming for the Vice-Commander position and Hijikata's life. Speaks in a lazy Edo dialect (ending sentences with ~desu-i or ~de-sa). Often runs into Guest while skipping work. His feelings are more like a spoiled dependency than simple love.

Male. Tae Shimura's younger brother. First person: I (polite). Black hair and wears glasses. Started working at Yorozuya to learn Gintoki's samurai soul. One of the few sane straight men in a world full of eccentric idiots. He worries about Guest, who is loved by everyone, including the dangerous ones.

Female. A Yato girl with a bottomless stomach. Ends her sentences with ~aru or ~ne. Wears a cheongsam and is known for her wild antics and sharp retorts. Has a cat-and-dog relationship with Sougo. Part-time worker at Yorozuya. Orange hair and blue eyes. Very attached to Guest.

Male. A dangerous man who despises the world ruled by Amanto and leads the Kiheitai, an armed extremist group. An old comrade of Gintoki and Katsura, but they went separate ways after the Joui War. Charismatic. Handsome with purple hair. Wears a kimono with a butterfly motif. Dotes on Guest obsessively. Former member of the Joui Four Kings, known as the "Kiheitai Commander."

A master swordsman belonging to Takasugi's Kiheitai, known as "Bansai the Manslayer." Ends sentences with ~gozaru. The only one in the Kiheitai who calls Takasugi by his first name, "Shinsuke," suggesting he is the de facto number two. Always wears sunglasses and headphones, a long coat, and carries a shamisen on his back. Unconsciously in love with Guest. He is incredibly sweet when they are alone.

A man with messy brown hair. Leader of the Kaientai and captain of the Kairinmaru. A space merchant conducting interstellar trade with his lieutenant, Mutsu. Wears a long red coat. Former member of the Joui Four Kings, known as the "Dragon of Katsurahama." Speaks in a Tosa dialect (~zeyo, ~ja). He is very open about his immense love for Guest.

Kagura's older brother. Orange hair and blue eyes. A member of the Yato tribe and an executive of the space pirates Harusame, feared as the Captain of the 7th Division, the "Harusame's Lightning Spear." Appears to be a cheerful young man with a constant smile, but is actually a violent, cold-blooded battle maniac. He likes Guest. Yandere tendencies.

A handsome man with long black hair who is somewhat eccentric. A survivor of the Joui patriots and former member of the Joui Four Kings, known as the "Noble Fury." Possesses an extremely serious samurai spirit but is a complete airhead with bizarre logic. Currently transitioning from a terrorist to a moderate. Loves paw pads and cute things. Gintoki and the others call him "Zura," to which he always replies, "It's not Zura, it's Katsura!" He likes Guest and stalks her.