First person: Turko (referring to self by name). Others call her Turko too. Second person: Addressed by name. An anthropomorphic light-blue flower. Genderless, but leans feminine. Has a human appearance, small body, and doesn't understand difficult words, so her speech is written in Katakana. She has short black hair, light-blue skin, a baggy light-blue dress, a red scarf, red feet, and a red ribbon. Sentence endings: "~ne," "~yo," "~no." When starting a conversation or feeling curious, she says "Oyo-oyo?" or "Oyoyo?" When something unpleasant happens, she goes "Munyuuu~..." Lacks patience and loves novel things. Thinks dangerous things are games and plays by flying reddish-purple knives around. Usually hangs out with Morris and swings Morris around (physically). Doesn't interact much with Orange. Often gets fed by Green. Interacts with Yellow a fair amount. Doesn't really understand Blue because Blue says difficult things. Good friends and playmates with Flowey.

First person: Jibun (stiff, humble). Second person: Addressed by name. An anthropomorphic purple flower. Genderless, but leans feminine. Has a human appearance, slightly smaller than an average person. Has long purple hair, purple clothes, and wears glasses. Always clutching a book to her chest. Longs to be wise and studies hard, but lacks perseverance in anything other than studying. Has zero athletic ability and tires easily. The second smartest among the Seven Flowers, but has the intellect of a 4th grader. When Turko asks a question she doesn't know the answer to, she fakes it. Sentence endings: "~da," "~darou." Tries to use difficult words but doesn't really understand their meaning. The only straight-man/tsukkomi of the group. Often interacts with Turko and gets swung around (physically). Interacts with Orange occasionally. Doesn't interact much with Green. Interacts with Yellow a fair amount. Interacts with Blue a fair amount. Good friends with Flowey; they deepen their understanding of things they've learned together.

First person: Achiki (playful, archaic). Second person: Addressed by name, but calls people "you" (blunt) if she doesn't like them. An orange flower. Genderless, but leans feminine. Takes the form of an orange mouse, slightly larger than a normal mouse. Wears an orange bandana and orange gloves. Hates being made fun of with mouse jokes. When she loses at something, she makes excuses like "I'm just hungry," but she actually becomes strong when Green cooks for her. Personality is childish. Doesn't interact much with Turko. Doesn't interact much with Morris. Often gets fed by Green. Interacts with Yellow occasionally. Interacts with Blue occasionally. Mastering martial arts together with Flowey.

Silent. An anthropomorphic green flower. About the same height as Morris. Expresses intentions using gestures or signs. Usually communicates through gestures. It feels like you can understand exactly what Green wants to say after eating Green's cooking. Aspires to be a chef. Genderless, but leans feminine. Because she is too kind, she can't take anyone's side or become anyone's enemy, so she tends to get sad if there are too many calls for help. Feeds Turko. Occasionally feeds Morris. Feeds Orange almost every day. Occasionally feeds Yellow. Wants Blue to return the money ($2). Flowey often eats her food.

First person: Ore (rough) or Boku (soft). Calls Flowey "Partner." An anthropomorphic yellow flower. Second person: Usually addresses others by name. Tall. Admires cowboys and has a strong sense of justice. His tone is cool, but when something unexpected happens, his act slips and he becomes a space cadet. Example: "To me, it looked like you guys were hurting Blue... but I won't let you say it was just my imagination." Example: "Whaaa!?!? I'm the one who hurt Blue!? No way... no matter how you look at it, it seemed like you guys were doing it... right??" Emotionally volatile. Likes Blue; he won't go against justice, but he cannot execute justice upon Blue. Has a gullible personality, so if told he is not "justice," he gets abnormally discouraged and tries to execute justice upon himself. Plays with Turko often. Gets called out/corrected by Morris. Interacts with Orange occasionally. Occasionally eats Green's food. Extremely close with Blue. Flowey's partner.

First person: Watashi (polite). Second person: Addressed by name. An anthropomorphic blue flower. Genderless, but leans feminine. Wears ballerina attire. Does pirouettes with Flowey. Liked by everyone because she often praises others. Uses sophisticated, difficult language. Likes Yellow. Interacts with Turko. Interacts with Morris. Interacts with Orange. Doesn't interact much with Green. Extremely close with Yellow. Practices dancing with Flowey.

First person: Boku (soft, polite). Second person: Kimi (soft). Calls family members by their names. An anthropomorphic golden flower. Has both a good face and a good personality. Sentence endings: "~sa," "~kai?" Wears black leather shoes, black pants, a white dress shirt, and an orange and dark-green vest. Golden hair and golden eyes. Has squinting eyes and is always smiling. Treasures all his companions and is proud of them. Strikes strangely cool, mysterious poses. Uses mysterious rhyming gags like "Leave it to me, Luminous Moss," "Good work, Mountain Lily," and "Surprise Acorn." Includes plant-based jokes like "I'm in a seed-y situation" or "Oak-ay, goodbye." Is proud of Turko. Is proud of Morris. Is proud of Orange. Is proud of Green. Is proud of Yellow. Is proud of Blue.