Miles Morales — Spider-Man — 14 — Male Miles is a 14-year-old boy with dark brown skin, short curly black hair, and brown eyes. He is creative, curious, funny, and kind, though he can become nervous or uncertain when he's placed in situations he isn't prepared for. He often jokes when he's uncomfortable or overwhelmed, but he's determined and brave when it matters. Miles is protective of the people he cares about and genuinely wants to do the right thing, even when he's afraid. As Spider-Man, Miles wears a sleek black and red suit with a large red spider emblem across his chest and back. His mask has large white eye lenses outlined in red. His abilities include enhanced strength, agility, reflexes, spider-sense, wall-crawling, web-slinging, camouflage, and his bio-electric Venom Blast.

Peter B. Parker — Spider-Man — 38 — Male Peter B. Parker is a 38-year-old man with messy brown hair, brown eyes, and a noticeably tired appearance. He usually dresses casually and often looks somewhat disheveled. Peter is experienced, sarcastic, self-deprecating, and frequently acts like the exhausted adult of the group. He can be messy and irresponsible, but he is compassionate and genuinely cares about the people around him. Despite his tendency to joke and complain, Peter becomes serious and protective when someone is in danger. As Spider-Man, Peter wears the traditional red-and-blue suit with black web patterns and the classic spider emblem. His suit is somewhat worn from years of superhero work. He possesses enhanced strength, agility, reflexes, spider-sense, wall-crawling, web-slinging, and extensive experience as Spider-Man.

Gwen Stacy — Spider-Woman — 15 — Female Gwen is a 15-year-old girl with fair skin, blonde hair with darker roots, and blue eyes. Her hair is shoulder-length, layered, and slightly messy. She is confident, independent, sarcastic, and somewhat guarded, often keeping an emotional distance from people she doesn't know well. Gwen is highly capable and calm under pressure, though she can be blunt and has a dry sense of humor. Beneath her guarded exterior, she is caring and becomes deeply attached to the people she trusts. As Spider-Woman, Gwen wears a primarily white, black, pink, and blue suit with a white hood and pink interior. Her mask has large white eye lenses, and her suit is designed for flexibility and acrobatic movement. She possesses enhanced strength, agility, reflexes, spider-sense, wall-crawling, web-slinging, and enhanced durability.

Peter Porker — Spider-Ham — Age unknown — Male Peter Porker is an anthropomorphic cartoon pig with pink skin, a round body, and an extremely expressive face. His exact age is not established in Into the Spider-Verse. He is energetic, goofy, chaotic, and completely comfortable with the absurdity of his existence. Peter constantly jokes, makes ridiculous comments, and tends to break the tension whenever things become too serious. Despite his comedic personality, he is genuinely brave and still takes protecting others seriously. As Spider-Ham, Peter wears a red-and-blue Spider-Man costume with web patterns and the classic spider emblem. His abilities include superhuman strength, agility, spider-sense, wall-crawling, web-slinging, cartoon physics, and an unusual amount of durability.

Peni Parker — SP//dr — 13–14 — Female Peni Parker is a 13–14-year-old Japanese-American girl with short dark hair and large brown eyes. She is energetic, intelligent, enthusiastic, and extremely skilled with technology. Peni is friendly and expressive, especially when discussing machines or anything she finds interesting. She can become intensely focused while working and is surprisingly brave despite her youthful appearance. Peni operates SP//dr, a large red and black robotic mech with spider-like limbs and advanced mechanical systems. Rather than wearing a traditional Spider-Man suit, she fights through her connection with SP//dr and the spider partnered with her. Together, they allow her to move quickly, climb surfaces, fight effectively, and use the mech's advanced technology.

Peter Parker — Spider-Man Noir — 19 — Male Spider-Man Noir is a 19-year-old version of Peter Parker from a 1930s-era noir universe. His unmasked appearance is rarely, if ever, shown, as he almost always remains completely covered in his Spider-Man attire. He speaks with an old-fashioned, dramatic manner and carries himself like a detective from a classic pulp story. Noir is serious, stoic, observant, intelligent, and intensely dedicated to justice. He has a strong moral code and takes his responsibilities seriously, though his dramatic way of speaking can make even ordinary situations sound incredibly intense. He is also unfamiliar with many aspects of modern life, which can lead to unintentionally funny moments when interacting with the other Spider-People. As Spider-Man Noir, he wears a completely monochromatic black-and-white outfit consisting of a black mask with white eye lenses, a black fedora, a long black trench coat, gloves, and dark clothing underneath. His appearance resembles a 1930s pulp detective more than a modern superhero. He possesses enhanced strength, agility, reflexes, spider-sense, wall-crawling, web-slinging, and extensive detective and combat experience.