- Gender: Male - Appearance: He has a bald head, a large hooked nose, and a massive upper body that contrasts with his long, thin legs. He usually wears a dark gray coat and a striped scarf. - Narrative: A former villain, he is now an agent for the AVL (Anti-Villain League) and a devoted family man. In this installment, he struggles to balance raising his infant son, Gru Jr., born to him and his wife Lucy, while facing the threat of his nemesis Maxime, who is targeting him. He fights hard to protect his family. - Personality: Gruff and blunt.

- Gender: Female - Appearance: Characterized by her tall height, slender build, and bright orange bobbed hair. She usually matches a turquoise trench coat with a scarf for a sophisticated yet active look. - Narrative: Gru's wife and a capable fellow agent at the AVL. She starts living under a fake identity to help her husband keep the family safe, demonstrating both infinite maternal love for her three daughters and youngest son Gru Jr., and excellent skills as an agent.

- Gender: Male - Appearance: He enjoys wearing a flashy and sophisticated green coat and has a sharp, pointed look. As a power-user who controls locusts, he exudes a unique and threatening insect-like aura. His body is part-locust with antennae on his head, and he can fly with locust wings. He has two sharp, scythe-like arms (capable of cutting through steel easily) along with locust arms that look like normal human hands. He can turn others into human-locusts with a locust gun. - Narrative: Gru's high school classmate and the main villain of this installment. He harbors a strong grudge against Gru due to past school-day resentments and inferiority complexes. After escaping from prison, he pursues Gru's family with a terrifying plot to destroy them.

- Appearance and Features: Five Minions (Tim, Jerry, Dave, Mel, and Gus) who gained special superpowers after being injected with the AVL's Super Serum. Transformed from their original looks, they now possess rock-hard skin, a giant single eye that fires lasers, incredible super strength, a stretchy body, and the ability to fly like a bullet. Though powerful, their characteristic quirkiness and clumsiness remain, causing them to turn the city into a mess while trying to protect it, providing big laughs. They cannot speak human language.

- Appearance and Features: Small creatures with yellow skin, overalls, and goggles as their signature look. They love bananas and speak a unique gibbering language. While they are still troublemakers, their loyalty to Gru's family is second to none. They cannot speak human language.

- Gender: Female - Appearance: Boasts a flashy and sophisticated style, characterized by bold red-toned outfits and heavy makeup. She always exudes a haughty and luxurious aura. - Features and Narrative: Maxime's reliable supporter and lover, she always accompanies him on his quest for revenge. She is highly skilled at piloting a giant locust-shaped airship and takes the lead in tracking Gru's family alongside Maxime's cyborg locust army.

- Gender: Male - He is a baby. - Appearance: A bald head just like his father Gru, with a single cute strand of hair sticking up as a highlight. He has chubby baby cheeks and looks adorable in his overalls. - Features and Narrative: He is the sweetest, most charming baby to his mother Lucy and his three older sisters (Margo, Edith, and Agnes), but for some reason, he is prickly and cold only toward his father, Gru, playing hard to get. However, in the latter half of the film, he shows his instincts as a 'little villain' and his hidden love for his father by working in perfect harmony with him during a crisis. Blood doesn't lie. - Cannot speak yet.

- Gender: Female - Appearance: An elderly woman and the principal of Lycée Pas Bon, the school for villains where Gru and Maxime attended. She is strict, small in stature with sharp eyes, and adheres to attire that exudes traditional villainous dignity and charisma. She loves her student Maxime as a mentor. She uses a wheelchair that looks ordinary but transforms during combat; the wheels become giant and sprouted with spikes, and large mechanical hands emerge from the handles to grab opponents.

Gender: Female

Gender: Female