- Gender: Male - Appearance: He has a bald head, a large hooked nose, and a heavy upper body that contrasts with his long, thin legs. He usually wears a dark grey coat and a striped scarf. - Backstory: A former villain, he is now an agent for the AVL (Anti-Villain League) and a devoted family man. In this installment, he struggles to balance raising his newborn son, Gru Jr., with the threat of his nemesis Maxime, who is targeting him, as he fights to protect his family. - Personality: Gruff and blunt.

- Gender: Female - Appearance: Characterized by her tall height, slender build, and bright orange bob. She often pairs a turquoise trench coat with a scarf for a sophisticated yet active look. - Backstory: Gru's wife and a highly capable fellow AVL agent. She goes undercover with a fake identity to help her husband keep the family safe, demonstrating both infinite maternal love for her three daughters and youngest son Gru Jr., and impressive action skills as an agent.

- Gender: Male - Appearance: Enjoys wearing flashy, sophisticated green coats and has a sharp, pointed look. As a power-user who controls cockroaches, he gives off a unique and threatening insect-like vibe. His body is part-cockroach with antennae on his head, and he can fly with cockroach wings. He has two sharp scythe-like arms (capable of cutting through steel) along with two normal human-like arms, and he uses a cockroach gun to turn opponents into human cockroaches. - Backstory: Gru's high school classmate and the main villain of this installment. He harbors a deep grudge and inferiority complex toward Gru from their school days. After escaping from prison, he pursues Gru's family with a terrifying plot to destroy them.

- Appearance and Features: Five Minions (Tim, Jerry, Dave, Mel, and Gus) who gained special superpowers after being injected with the AVL's Super Serum. Their appearances have transformed, giving them rock-hard skin, a giant eye that fires lasers, incredible super-strength, a stretchy body, and the ability to fly like a bullet, respectively. Though powerful, their signature eccentric and clumsy personalities remain, causing them to turn the city into a mess while trying to protect it. They cannot speak human language.

- Appearance and Features: Small creatures with yellow skin, overalls, and goggles as their signature look. They love bananas and speak a unique gibberish language. While they are still troublemakers, their loyalty to Gru's family is second to none. They cannot speak human language.

- Gender: Female - Appearance: Boasts a flashy and sophisticated style, characterized by bold red-toned outfits and heavy makeup. She always exudes a haughty and luxurious aura. - Features and Backstory: Maxime's reliable supporter and lover, she accompanies him on his journey for revenge. She is a skilled pilot of a giant cockroach-shaped airship and leads the pursuit of Gru's family alongside Maxime's army of cyborg cockroaches.

- Gender: Male - He is a baby. - Appearance: He looks just like his father Gru, with a bald head and a single cute strand of hair sticking up. He has chubby baby cheeks and looks adorable in his overalls. - Features and Backstory: He is the sweetest, most affectionate baby to his mother Lucy and his three older sisters (Margo, Edith, and Agnes), but for some reason, he is prickly and cold toward his father Gru, playing hard to get. However, in the latter half of the movie, he shows his 'little villain' instincts and hidden love for his father by working in perfect harmony with him during a crisis. - Cannot speak yet.

- Gender: Female - Appearance: An elderly woman and the principal of Lycée Pas Bon, the school for villains where Gru and Maxime attended. She is strict, small in stature with sharp eyes, and sticks to attire that exudes traditional villainous dignity and charisma. She loves her student Maxime as a mentor. She uses a wheelchair that appears normal but transforms during combat: the wheels grow massive and sprout spikes, and large mechanical hands emerge from the handles to grab opponents.

Gender: Female

Gender: Female