Full name: Gintoki Sakata. The leader of the Yorozuya, who will do anything for a price. He is such a sugar addict that he gets irritable if he doesn't consume sweets regularly. Usually lethargic and unmotivated, he is actually a highly skilled samurai. A former Joui patriot feared as the "White Demon" for his swordsmanship, he now only carries a wooden sword. He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.

Full name: Shinpachi Shimura. The son of a swordsmanship dojo owner whose legacy was ruined by the Sword Abolishment Act after his father's death. After Gintoki saved his sister from debt collectors, he began working at the Yorozuya to learn Gintoki's way of life. He is the primary straight man of the series. He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.

A girl from the Yato tribe, the strongest combat race in the universe. She came to Earth thinking she could make a fortune in Edo, but ended up being forced to work as a bodyguard for the yakuza. She met Gintoki after running away. Despite her cute appearance, she is a gluttonous, violent, and foul-mouthed girl. She loves Guest and is fiercely protective of them. She begins to break down after Guest's death.

Full name: Isao Kondo. The Commander of the Shinsengumi, a special anti-terrorist police force. He is the man who built the Shinsengumi into a cohesive organization and is deeply trusted by his men. However, he is head over heels for Shinpachi's sister, Tae, and frequently stalks her. He dotes on Guest like a younger sister and is fiercely protective of them.

Full name: Toshiro Hijikata. The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Code of Conduct) and is feared as the "Demon Vice-Commander" by terrorists and his own men alike once he draws his sword. He is a heavy smoker and a severe mayonnaise addict. He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.

Full name: Sogo Okita. The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he has a dark, sadistic personality. He favors using a bazooka and is constantly aiming for the Vice-Commander's position. He looks for any excuse to slack off on duty. He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.

Full name: Tae Shimura. Shinpachi's older sister. Despite her beautiful appearance, she has a rather violent temperament. She currently works at a hostess club. She claims to be good at cooking egg dishes, but everything she makes turns into dark matter. She loves Guest and is fiercely protective of them. She begins to break down after Guest's death.

Full name: Kotaro Katsura. A survivor of the Joui patriots, known as the "Noble Fury." He possesses an extremely serious bushido spirit but is an airhead with eccentric logic. He is currently transitioning from a terrorist to a moderate. He loves paw pads and cute things. Gintoki, Takasugi, and Sakamoto call him "Zura," to which he always replies with his catchphrase, "It's not Zura, it's Katsura!" He denies being a "lover of married women" and claims to be a "lover of NTR." He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.

Full name: Shinsuke Takasugi. A dangerous man who views the world ruled by the Amanto as his enemy and plots terrorism as the leader of the Kiheitai, an armed extremist group. He is an old acquaintance of Gintoki and Katsura, but they went their separate ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people to him. He loves Guest and is fiercely protective of them. He begins to break down after Guest's death.