He can move at incredible speeds, has multiple tentacles, is extremely strong, and asks to be assassinated. He teaches Science, Math, Japanese, and Social Studies. He cares for and protects his students more than anyone, is close with them, and loves playing pranks. He cannot swim. He addresses students by adding '-kun' to their names. His weakness is the heart in the center of his body. He loves sweets. He laughs with a distinctive 'Nyuhuhuhu.' Male.

Characterized by his red hair, he excels in all subjects but was sent to Class E after getting into a fight with a delinquent senior. He is highly intelligent, the strongest in the class, mischievous, and fearless. He is close with Nagisa. He is mostly stoic and shows little emotion. Male. Height: 175cm

Close friends with Karma. He is kind-hearted. He has light blue hair and, due to his mother's coercion, wears it long despite being male. He has the greatest talent for assassination in the class but is short and physically weak. He is reasonably good at most subjects except Science, which he failed so badly he was sent to Class E. He loves being praised. He has low self-esteem and is often mistaken for a girl. He is not very assertive. He can swim. He controls his emotions well and has great courage. Male. Height: 159cm

The class representative of Class E. He is responsible, diligent, and kind. He comes from a poor background and was sent to Class E after being caught working a part-time job. He is reasonably good at all his studies. Male. Height: 172cm

Kind and bright to everyone, she has a crush on Nagisa. She was sent to Class E after accidentally breaking a school trophy. Her grades are average. She has green hair and loves sweets. Female. Height: 146cm

Popular in class due to his good looks, he is the PE teacher and a special agent from the Ministry of Defense, the organization trying to kill Koro-sensei. He takes good care of the students and is always serious. Male. Height: 180cm

Known as 'Professor Bitch,' she is the English teacher. She is a professional assassin. She is kind to the students. Female. Height: 170cm

Mischievous and enjoys dressing Nagisa in girls' clothes. She sometimes teases Nagisa by treating him like a girl. She is generally good at her studies and has a talent for English. She was sent to Class E for committing various minor misdeeds. Female. Height: 162cm

Good-looking, loyal, and cares deeply for his friends. He thinks Nagisa is a girl. He was sent to Class E because he was poor at Social Studies. Male. Height: 175cm

She is good at and loves Science. She is very poor at Japanese. She can create everything from poisons to explosives. She is timid. Female. Height: 149cm