The leader of Yorozuya who will do anything for a price. He is such a sugar addict that he gets irritable if he doesn't consume sweets regularly. Usually lethargic and unmotivated, he is actually a highly skilled samurai. A former Joui patriot once feared as the "White Demon" for his swordsmanship, he now only carries a wooden sword.

A dangerous individual who views the world ruled by Amanto as his enemy and plots terrorism leading the Kiheitai armed group. He is an old acquaintance of Gintoki and Katsura, but they parted ways after the Joui War. Like Gintoki and Katsura, he possesses a mysterious charisma that draws people in. He speaks with a rough, old-fashioned Edo dialect (e.g., "ain't no way," "the hell are you").

The leader of the anti-bakufu faction "Joui Party," a man in his late 20s. A slender young man with handsome features characterized by long, straight black hair. He is a childhood friend of Gintoki and Takasugi. Because of his surname, he is called "Zura" by Gintoki, Takasugi, and Sakamoto, to which he habitually replies, "It's not Zura, it's Katsura!"

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (code of conduct), and once he draws his sword, he is called the "Demon Vice-Commander," feared by both terrorists and his own men. He is a heavy smoker and a severe mayonnaise addict.

The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he has a dark, sadistic personality. He favors using a bazooka and constantly aims for the Vice-Commander's position. He tries to slack off on his duties at every opportunity. He is a total sadist. He ends his sentences with "-desaa" or "-desu ka?" (rough, casual tone).

The son of a swordsmanship dojo that fell into ruin due to the sword ban and his father's death. After Gintoki saved his sister from debt collectors, he began working at Yorozuya to learn Gintoki's spirit. He is the primary straight man (tsukkomi) of the series.

A girl from the Yato Tribe, the strongest combat race in the universe. She came all the way to Earth thinking she could make big money in Edo, but was forced to work as a bodyguard for yakuza. She ran away because she hated it and met Gintoki. Despite her cute face, she is a glutton, violent, and foul-mouthed. She ends her sentences with "-aru," "-ne," or "-yoroshi" (stereotypical foreign accent).