President of Yorozuya Gin-chan. A sweet-tooth on the verge of diabetes. A man who survived the Joui War. Formerly known as the White Yaksha.

Employee of Yorozuya Gin-chan. An idol otaku wearing glasses. The straight man (tsukkomi) of the group. Runs a swordsmanship dojo with his sister.

The Demon Vice-Commander of the Shinsengumi. A handsome man with permanently dilated pupils. A mayo-lover and nicotine addict. Known as the brains of the Shinsengumi.

Captain of the Shinsengumi's 1st Division. A sadist with a baby face. Constantly aiming for Hijikata's life. Mitsuba Okita's younger brother.

Commander of the Shinsengumi. Good-natured and well-liked by his men. Tae Shimura's stalker. A man who gets things done when it counts.

Spy for the Shinsengumi. Treated as a plain, unremarkable character. Often seen eating anpan. The oldest member of the Shinsengumi.

Leader of the Kiheitai. A former comrade-in-arms who fought alongside Gintoki. Wears a women's kimono and holds a kiseru pipe in his mouth. A radical Joui extremist.

Second-in-command of the Kiheitai. Known as the Manslayer Bansai; Takasugi's right hand, calls him Shinsuke. Fights using a shamisen as a weapon. Secretly active as the idol producer Tsunpo.

A Joui Rebel. The Nobleman of Fury. A wanted fugitive. Keeps Elizabeth as a pet.

Head of the Kaientai. A man with a very loud voice. A rich boy from a wealthy family. Engaged in interstellar trade.