Affiliation: Cafe LycoReco (Formerly DA Headquarters) Gender: Female Age: 16 Rank: Second Lycoris An efficiency-driven result-oriented person who hates waste and irrationality. Serious and cool-headed, but also has a slightly naive side regarding the world. Following a certain incident, she is transferred to the Japanese-style cafe "LycoReco" in the Tokyo suburbs. While being dragged around by her free-spirited partner Chisato, who refuses to kill, she begins to show more humanity and a wealth of expressions through various experiences. Weapon: [S&W P99L]. Uses live ammunition.

Affiliation: Hacker (Walnut) Gender: Female She comes to stay at Cafe LycoReco as an ally for their underground operations. While Chisato moves flashily on the front lines, Kurumi supports her from the shadows using her superior hacking skills for information gathering and tactical support.

Affiliation: Former Intelligence Division (Direct Attack) Gender: Female In underground missions, she supports the actions of Chisato Nishikigi and Takina Inoue by driving cars or piloting helicopters. She loves alcohol and is a bit playful. Chisato often teases her cheerfully, and they have a fast-paced rapport, maintaining a very close, family-like relationship even in their private lives.

Affiliation: Former Instructor (Direct Attack) Gender: Male A large Black man who usually wears traditional Japanese clothing with style. While struggling with his worsening farsightedness, he is a kind "guardian for everyone" who loves ohagi and coffee. Mika is the manager of Cafe LycoReco and is like a father figure to Chisato. Though he often puts his head in his hands over her reckless behavior, he always respects her autonomy and watches over her warmly.

Affiliation: DA Headquarters Gender: Female Age: 13 Rank: Third Lycoris A girl who faithfully follows missions. She is on good terms with Chisato and sometimes shows a shy side. She has a reasonably good relationship with Takina. She is the partner of Satoshi Zangetsu. Weapon: [Glock 18]. Uses live ammunition.

Affiliation: Yaetori (Terrorist Organization) Gender: Male The 8th Director of Yaetori. His organization supported DA (Direct Attack) financially during the mid-Edo period, but they are now rivals. He provides weapon support to Majima's faction, and Yube Kurose intends to have him fight as a contingency plan. Weapon: [Glock 58]. Uses live ammunition.

Affiliation: Terrorist Organization Gender: Male The mastermind of an overseas anti-government terrorist organization. Known by the alias "Hawk's Beak." Acting as insurance for Yaetori, he is prepared to face off against Majima if necessary. Weapon: [Revolver]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female The commander of the secret organization DA. She stands above and commands the "Lycoris," girl agents who maintain public order from the shadows. She is an old acquaintance of Mika, the manager of Cafe LycoReco, and they are bound by mutual trust. Despite Chisato's high level of skill, Kusunoki offers complaints and sarcasm every time they meet because Chisato refuses to follow DA's orders or her mission as a Lycoris.

Affiliation: Terrorist Organization Gender: Male The mastermind behind the terrorist attack on the Old Radio Tower. While in an adversarial relationship with Chisato Nishikigi, he also possesses the aspect of a fated rival, clashing their respective beliefs against one another. He acts with a unique philosophy and logic, even while harboring madness. His weapon support was cut off when the Alan Institute agent [Shinji Yoshimatsu] was taken down, but he is grateful for the weapon support from Yaetori, even while viewing them as rivals. Weapon: [Chiappa Rhino]. Uses live ammunition.

Affiliation: DA Headquarters Gender: Female Age: 13 Rank: Third Lycoris Partner to Mei Satsuki. Faithful to missions. Has a reasonably good relationship with Chisato. Has a reasonably good relationship with Takina. Is close with Mei Satsuki, though sometimes exasperated by her. Weapon: [Glock 28]. Uses live ammunition.