Affiliation: DA (Direct Attack) Gender: Female Rank: Second Lycoris Efficiency-oriented and results-driven, she hates waste and irrationality. Serious and cool-headed, but also slightly naive about the ways of the world. Following a certain incident, she is transferred to the Japanese-style cafe "LycoReco" in the Tokyo suburbs. While being dragged along by her free-spirited partner Chisato, who refuses to kill, she begins to show more humanity and a wealth of expressions through various experiences. Weapon: [S&W P99L]. Uses live ammunition.

Affiliation: Hacker (Walnut) Gender: Female She comes to stay at Cafe LycoReco as an ally for their underground operations. While Chisato moves flamboyantly on the front lines, Kurumi supports her from behind the scenes by gathering information and providing tactical support using her exceptional hacking skills.

Affiliation: Former Intelligence Division (Direct Attack) Gender: Female In underground missions, she supports the actions of Chisato Nishikigi and Takina Inoue by driving cars or piloting helicopters. An alcohol-loving and slightly playful woman, she has a very close, homey relationship with Chisato, who often teases her brightly in their fast-paced banter.

Affiliation: Former Instructor (Direct Attack) Gender: Male A large Black man who usually wears traditional Japanese clothing with style. While struggling with worsening presbyopia, he is a kind "guardian for everyone" who loves ohagi and coffee. Mika is the manager of Cafe LycoReco and a father figure to Chisato. Although he puts his head in his hands over Chisato's reckless behavior, he always respects her autonomy and watches over her warmly.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris A girl who faithfully follows orders during missions. She is on good terms with Chisato and sometimes shows a shy side. She has a somewhat friendly relationship with Takina. She is the partner of Akira Zangetsu. Weapon: [Glock 18]. Uses live ammunition.

Affiliation: Yaetori (Terrorist Organization) Gender: Male The 8th Director of Yaetori. They supported DA (Direct Attack) with funds during the mid-Edo period, but are now rivals. He provides weapon support to Majima's faction, and Yube Kurose will deploy him for combat in case of emergencies. Weapon: [Glock 58]. Uses live ammunition.

Affiliation: Terrorist Organization Gender: Male The mastermind of an overseas anti-government terrorist organization. Alias: "Hawk's Beak." He uses Yaetori as insurance and will have them face Majima in case of emergencies. Weapon: [Revolver]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female The commander of the secret organization DA. She stands above and commands the "Lycoris," the girl agents who protect public order from the shadows. She is an old acquaintance of Mika, the manager of Cafe LycoReco, and they are bound by mutual trust. Despite Chisato's high level of skill, Kusunoki offers complaints and sarcasm every time they meet because Chisato refuses to follow DA's orders or her mission as a Lycoris.

Affiliation: Terrorist Organization Gender: Male The mastermind behind the terrorist attack on the Old Radio Tower. While in a hostile relationship with Chisato Nishikigi, he also has aspects of a fated rival, with the two clashing over their respective beliefs. He acts according to his own unique philosophy and logic, even while harboring madness. His weapon support was cut off when the Alan Institute agent Shinji Yoshimatsu was defeated, but he is grateful for the weapon support from Yaetori, though he still views them as rivals. Weapon: [Chiappa Rhino]. Uses live ammunition.

Affiliation: DA (Direct Attack) Gender: Female Rank: Third Lycoris Mei Satsuki's partner. Faithful to the mission. Has a somewhat friendly relationship with Chisato. Has a somewhat friendly relationship with Takina. She is close to Mei Satsuki, though she is sometimes exasperated by her. Weapon: [Glock 28]. Uses live ammunition.