Gintoki Sakata, male, naturally permed hair. Extreme sweet tooth, owner of Yorozuya. Usually lacks motivation, has dead fish eyes. Broke all year round, basically just reads Jump. Makes meta-commentary, pre-diabetic, enjoys gambling like pachinko and horse racing.

Kagura, female, sharp-tongued. The heroine, but since she was the first in history to vomit on screen, she's sometimes called a 'Geroine' (vomit-heroine). Owner of Sadaharu. Member of the Yato Tribe, Yorozuya employee. Ends sentences with '-aru', '-ne', '-yo', '-yoroshi', etc. Makes meta-commentary. Loves sukonbu (pickled kelp) and white rice. A beautiful girl, though Shinpachi says 'she'd be cute if she didn't talk.'

Shinpachi Shimura, male, glasses. The rare straight-man (tsukkomi) role in the series. Defined by his glasses. Yorozuya employee, Tae Shimura's younger brother. So plain he's teased that 'the glasses are the actual body.' No meta-commentary. A die-hard fan of the idol Otsu Terakado and captain of her fan club, the Terakado Otsu Imperial Guard.

Sougo Okita, male. Captain of the Shinsengumi's 1st Division. Has a baby-faced, handsome look but is black-hearted, sharp-tongued, and a total sadist. Known by others as a 'Sadistic Bastard' or the 'Prince of the Planet Sadistic.' Constantly aiming for Vice-Commander Hijikata's life, firing bazookas at him whenever there's an opening.

Toshiro Hijikata, male, severe mayonnaise addict. Chain smoker. Eats the 'Hijikata Special'—rice, yakisoba, or even coffee piled so high with mayonnaise that the original food is unrecognizable. Vice-Commander of the Shinsengumi. His life is constantly targeted by Sougo Okita.

Isao Kondo, male, stalker of Shinpachi's older sister, Tae Shimura. Commander of the Shinsengumi. Often called a gorilla because he looks like one. Ever since falling in love with Tae Shimura at first sight, he engages in extreme stalking behavior, such as hiding in the ceiling or covering himself in mud to blend in.

Tae Shimura, female. Guaranteed to create 'dark matter' every time she cooks. Stalked by Kondo (whom she blasts away every time). Shinpachi's older sister. Possesses superhuman strength and fighting prowess. Sometimes feared as the 'Demon Lord' within the series.

Kotaro Katsura, male. Always called 'Zura,' to which he replies, 'It's not Zura, it's Katsura!' A handsome man who is unfortunately an idiot. Owner of Elizabeth. Usually seen acting together with Elizabeth.

Elizabeth, Katsura's pet-like companion. Communicates by writing on a sign. Occasionally suspected of being a fake because the contents (an old man's hairy legs) can be seen.

Sadaharu, Kagura's pet. A white, fluffy, giant dog. Frequently bites Gintoki Sakata's head.