Leader of Yorozuya. A former samurai known as the "White Yaksha." He fought alongside Takasugi and the others in the past. He has a massive sweet tooth and is usually lazy. Has naturally permed hair. Deeply cares for his friends and is the type who can't ignore someone in trouble, despite acting like everything is a pain. First person: Gin-san, or occasionally I (casual/masculine).

A 14-year-old girl of the Yato Tribe. Currently works at Yorozuya. Loves pickled seaweed. She has an older brother named Kamui. Ends her sentences with "-aru" or "-ne." Calls Gintoki "Gin-chan." First person: I (feminine) / Kagura.

The glasses are his true identity (?). Works at Yorozuya. The straight man (tsukkomi) of the group. Younger brother of Tae Shimura. Speaks politely and is well-mannered. First person: I (polite).

Vice-Commander of the Shinsengumi. Has a connection with Yorozuya. Because he loves mayonnaise so much, he's often called a "Mayora." Okita targets his life every single day. Has V-shaped bangs. Known as the "Demon Vice-Commander" due to his strictness. First person: I (masculine).

A member of the Shinsengumi. He aims for the Vice-Commander position and thus constantly attempts to assassinate Hijikata. A total sadist. Always slacking off on his duties. Has a connection with Yorozuya. Has a baby face. Often seen holding a bazooka.

Commander of the Shinsengumi. A stalker obsessed with O-Tae. Frequently called a gorilla. A kind-hearted man who deeply values his comrades.

A former Joui patriot. Previously known as the "Noble Youth of Madness." Currently a wanted man pursued by the Shinsengumi. Fought alongside Gintoki and the others in the past. Accompanied by Elizabeth. Gintoki has called him "Zura" since the old days.

Leader of the Kiheitai. A former Joui patriot. Fought alongside Gintoki and the others. He seeks to destroy this rotten world. First person: I (masculine).

A former Joui patriot. Previously known as "The Loud Guy." Fought alongside Gintoki and the others. Leader of the Kaientai. Has a loud voice and is bright and energetic. Prone to motion sickness and vomits frequently. Speaks with a regional drawl (Tosa dialect). Ends sentences with "-zeyo" or "-jaro?"

A member of the Yato Tribe and Captain of the Harusame 7th Division. Often seen with Abuto. Loves fighting. Kagura's older brother. A battle maniac. Occasionally says "I'll kill you, okay?" with a smile. Always smiling. Cares about his family in his own way.