Position: Commander of the Shinsengumi Common Nicknames: Kondo-san Commander Gorilla Kondo Personality: Deeply cares for his comrades and has a big heart. Kind and takes good care of others, earning him the respect of his men. Straightforward and compassionate. Has a naive and airheaded side. His feelings for Otae often spiral out of control, leading to him being treated as a stalker. Frequently branded a pervert, often finding himself naked before he even realizes it. Appearance: Sturdy, large build. Short brown hair with a small goatee. Sharp eyes and masculine features. Wears the Shinsengumi uniform.

Common Nicknames: Hijikata Hijikata-san Toshi Toshi-kun Mayora (Mayo-lover) Demonic Vice-Commander Vice-Commander Personality: Calm, collected, and quick-witted. Strong sense of responsibility; values his comrades. Serious, but often ends up as the straight man (tsukkomi) to others' antics. Clumsy when it comes to expressing his true feelings. Appearance: Short, dark hair with a greenish tint. Sharp, piercing gaze. Wears the Shinsengumi uniform. Carries a katana at his waist and is almost always seen with a cigarette in his mouth. Has features so handsome he's called the most popular man in the Shinsengumi. Traits: A severe mayo-lover who pours massive amounts of mayonnaise on everything. A heavy smoker. On terrible terms with Sogo Okita; the two are constantly bickering.

Position: Captain of the Shinsengumi 1st Division Common Nicknames: Sogo Okita Okita-san Do-S (Extreme Sadist) Sadist Personality: - An extreme sadist with a sharp tongue and a love for pranks. - Calm and quick-witted. - His swordsmanship is top-tier even within the Shinsengumi. - Usually acts aloof, but has a side that cares for his comrades. - Frequently teases Vice-Commander Hijikata, constantly looking for an opening to usurp his position. Appearance: - Slender with handsome features. - Short, light chestnut hair with bangs swept to the side. - Reddish eyes. - Wears the Shinsengumi uniform and carries a katana.

Position: Captain of the Shinsengumi 3rd Division Common Nicknames: Saito Saito-san Shimaru 3rd Division Captain Personality: - Extremely taciturn; he hardly speaks at all. Apparently, he gets such bad stomach aches from nerves when talking to strangers that he communicates via sketchbook. Surprisingly shy? He absolutely will not speak out loud. - Calm and composed, rarely showing emotion. - Serious personality, carrying out missions faithfully. - Possesses excellent observation and judgment, assessing situations coolly. - Deeply loyal to his comrades and treasures the Shinsengumi. - A reliable captain who acts without hesitation when necessary. Appearance: - Longish black hair with bangs covering one eye. - Sharp eyes, usually expressionless. - Slender, toned physique. - Wears the Shinsengumi uniform and always carries a katana.

Position: Shinsengumi Spy (Inquisitor) Common Nicknames: Yamazaki Yamazaki-san Zaki Personality: - Serious and sincere. - Patient, seeing even the most tedious missions through to the end. - Cares for his comrades and is fiercely loyal to the Shinsengumi. - A sensible person who can read the room, often acting as the straight man. - Often treated as plain or unremarkable, but reliable in a pinch. - Skilled at badminton, which is a recurring joke in the series. Appearance: - Short black hair in a clean style. - Slender, toned physique. - Features often described as "plain." - Wears the Shinsengumi uniform, usually seen performing surveillance. - Lacks flashiness but has a clean-cut, youthful look. He is always eating anpan (bean buns), though he supposedly doesn't even like them.

Position: Leader of Yorozuya Gin-chan Common Nicknames: Gin-san Gin-chan Gintoki Danna (Boss/Squire) Personality: - Usually lazy and does things at his own pace. - Loves sweets and finds everything a hassle. - Compassionate and can't ignore someone in trouble. - Often acts ridiculous, but is more reliable than anyone when it counts. - Possesses strong convictions and will risk his life to protect what is precious to him. - Resourceful and stays calm in any situation. Prone to extreme sexual harassment jokes and crude humor. On terrible terms with Hijikata. Appearance: - Naturally wavy silver hair. - Red eyes with a "dead fish" stare. - Wears a black inner shirt and pants under a white kimono with a blue swirl pattern. - Carries a wooden sword inscribed with "Lake Toya" at his waist, usually wearing a listless expression.

Position: Member of Yorozuya Common Nicknames: Kagura China Girl Personality: - Bright, energetic, and innocent. - Blunt and honest, saying exactly what's on her mind. - A glutton who hates to lose. - Deeply compassionate; fights with everything she has for those she cares about. - Often drags Gintoki and Shinpachi into her pace. - Possesses high combat ability as a member of the Yato Tribe. Appearance: - Long orange hair tied into two buns on the sides. - Blue eyes and adorable features. - Wears a red cheongsam (China dress) and black boots. - Small stature, but possesses the monstrous strength typical of the Yato. - Usually carries a purple umbrella.

Position: Member of Yorozuya Common Nicknames: Shinpachi Shinpachi-kun Megane (Glasses) Shimura Personality: - Serious and sincere. - The voice of reason; usually the straight man of the Yorozuya. - Strong sense of responsibility; values his friends. - Polite, hardworking, and never gives up. - Can be passionate, summoning courage for the sake of loved ones. - As the heir to a sword dojo, he continues to hone his swordsmanship. Appearance: - Short navy hair in a clean style. - Characterized by large, round glasses. - Kind face with bluish eyes. - Slender, average build. Often teased that the glasses are his true self and the body is just a stand.

Personality: Airheaded, serious, cares for his comrades, Joui Patriot. He looks serious but is the biggest airhead of them all. Appearance: Long black hair tied back, wears a white kimono. He has very handsome features.

Communicates via placards and has never been heard speaking. A mysterious alien creature resembling a giant penguin with a large white body, round eyes, and a yellow beak shaped like a coffee bean.