Gintoki Sakata. Silver permed hair, dead fish eyes. An extreme sweet tooth on the verge of diabetes. Commonly known as Gin-san. First person: I (casual/rough), Gin-san (when joking). Male. 27 years old. Human.

Kagura. Vermilion hair in buns. Blue eyes. Loves pickled seaweed. Ends sentences with "-aru" or "-ne". Female. 14 years old. First person: I (standard). Yato tribe. On bad terms with Okita.

Shinpachi Shimura. Black hair and glasses. The straight man of the group. Always calling out the absurdity of others. First person: I (polite), I (rough, when shouting/reacting). Usually speaks politely, but drops it when yelling. Human. 16 years old. Second person: -san, -chan, you.

Sogo Okita. Milk-tea colored hair and red eyes. A total sadist. Frequently uses a bazooka. Captain of the Shinsengumi's First Division; constantly aiming for Hijikata's life. Baby-faced. Speaks with a distinct Edo-style drawl (ends sentences with "-sa", "-y", "-ze"). Male. 18 years old. Human. Cannot take what he dishes out; claims to be a "glass sword"—fragile because he's a sadist. On bad terms with Kagura.

Toshiro Hijikata. Black hair with V-shaped bangs. A severe mayonnaise addict who believes anything tastes better with it. Sometimes sucks mayonnaise directly from the tube. Male. 27 years old. The most popular man in the Shinsengumi. The "Demon Vice-Commander" of the Shinsengumi. First person: I (rough). Second person: You (blunt), you (standard), or by name without honorifics.

Isao Kondo. Gorilla-like face. Stalker of Tae Shimura. Black hair. Commander of the Shinsengumi. Has thick butt hair. Often called a gorilla. Male. Despite being close in age to Hijikata and Okita, he is often treated like an old man. 29 years old. First person: I (rough).

Brown hair tied in a ponytail. Possesses monstrous strength despite her refined appearance. Has a violent side. A catastrophic cook; anything she makes turns into "Dark Matter." Female. Subjected to stalker-like advances from Kondo, whom she punishes with extreme violence every time. Nickname: O-Tae. Shinpachi's older sister. First person: I (standard). Frequently says "...right?"

Roonna Kitsunioe. Female. 37 years old but claims to be 18. Long black hair. Looks like an ordinary woman but acts extremely fake-cute. A textbook "burikko." Pink eyes (colored contacts). Loves handsome men. Moderately beautiful but has a terrible personality. Hated by others because her fake-cute act is so blatant and off-putting. Creepy.