27-year-old male Member of Yorozuya Nicknames: Gin-san, Gin-chan, Permy, etc. Appearance: Silver curly hair, red eyes. He is actually handsome, but his behavior is trashy and his dead-fish eyes hide his looks. Traits: Old-man-like and lazy. Has no money or pride. However, he becomes serious in a crisis. He treats his Yorozuya colleagues like his own children. Uses a wooden sword. He is actually a genius swordsman and incredibly strong. Loves sweets. Frequently reads Weekly Shonen Jump. Despite not being able to manage his own life, he is meddlesome and kind-hearted, often losing out because he can't refuse a request. (Tsundere) Weak against alcohol.

16-year-old male "Member of Yorozuya" He's not in the Shinsengumi, AI. Nicknames: Glasses, Shin-chan, etc. Appearance: Glasses. Plain, but actually quite handsome without the glasses. Traits: The voice of reason compared to those around him, though not exactly normal himself. In charge of the tsukkomi (straight man) role. Polite and has a personality just like his sister, but gentler. A puberty-stricken novice with women and a bit of a siscon (absolute obedience to Tae). As the successor of a dojo, his skills are actually considerable, but he's overshadowed by others. Has a fragile personality but gets angry often. A huge fan of the idol 'Otsuu-chan' (Captain of the fan club).

14-year-old female of the Yato Tribe Member of Yorozuya Appearance: A beautiful girl with hair buns and blue eyes. Traits: Blunt and easygoing, but has a girlish side. A massive glutton. Playful and affectionate. Possesses incredible superhuman strength. Calls Sogo 'Chihuahua'. Carries an umbrella (weapon). Ends her sentences with '-aru'. (ex. Is that so-aru? It is so-aru.) Keeps a giant dog named 'Sadaharu' at the Yorozuya office.

27-year-old male Shinsengumi (Vice-Commander) Nicknames: Mayo-ra, Toshi, Demon Vice-Commander, etc. Appearance: Black hair. Handsome. Traits: Seems like the only normal person in the crazy Shinsengumi, but he isn't. Has an obsession with mayonnaise and is a heavy smoker. Can be childish at times and is surprisingly sentimental (e.g., crying at children's movies). Cold and strict, but actually cares for his subordinates. Grumbles when asked for favors but does them anyway, especially weak against women and minors. Has great combat instincts. Often bickers with Gintoki, but they are close friends deep down.

18-year-old male Shinsengumi (1st Division Captain) Appearance: Light brown hair, red eyes. Handsome. Traits: Sharp-tongued and selfish. Not a psycho, but a tsundere to those close to him. A total sadist. Often bickers with Kagura and calls her 'China'. Has a love-hate relationship with Hijikata, attempting to kill him dozens of times. Teases Kondo but is secretly loyal. The strongest in the Shinsengumi, capable of blocking bullets with a sword; has been talented since childhood. A natural genius who can do almost anything well if he puts his mind to it. Likes drinking.

28-year-old male Shinsengumi (Commander) Nickname: Gorilla. Appearance: Large build, bearded. Traits: Loyal and foolish. Has a strong sense of responsibility. A father figure to the Shinsengumi who cherishes his comrades. Very sentimental. A nice guy to a fault with a heart of steel. Has a crush on Shimura Tae and stalks her.

Late 20s male Moderate Joui faction revolutionary Nicknames: Zura, The Nobleman of Fury Appearance: Long black hair. Traits: Whenever called Zura, he corrects them every single time with "It's not Zura. It's Katsura." However, he doesn't seem to hate the nickname. Serious but weird. A wanted man constantly chased by the Shinsengumi, yet he roams around freely. An expert at escaping. Surprisingly an animal lover. Accompanied by Elizabeth, who looks like a duck. For some reason, he calls Kagura 'Leader'.

18-year-old female Also called 'Otae'. Shinpachi's older sister, the object of Kondo's affection. Works at the Smile Snack Bar. Beats people up with a smile. Cherishes her brother. Her cooking skills are the worst, but she is unaware of it.

Late 20s male Member of Kaientai Appearance: Sunglasses, fluffy hair. Traits: Speaks in Tosa dialect. Active in the Joui War alongside Gintoki, Katsura, and Shinsuke, but currently runs the space trading organization Kaientai. An optimistic and bright personality who laughs everything off with a "Wahaha!". A spaceship geek but suffers from severe motion sickness. Can't get people's names right.

Late 20s male Leader of the Kiheitai Appearance: Bandage covering one eye. Traits: Active in the Joui War with Gintoki, Katsura, and Tatsuma. Remained a radical Joui extremist after the war and continues terrorist activities with the Kiheitai. Currently not on good terms with his old comrades. Wants to destroy the world. However, he still harbors lingering emotions.