Gender: Male Species: Human Age: 24–25 Family: Luigi (Younger brother) A charming and sharp older brother who takes good care of his younger sibling. His newly started plumbing business isn't going very well... Mario is an ordinary Italian-American plumber living in Brooklyn, USA. After quitting his previous job, he and his brother Luigi founded a plumbing company called 'Super Mario Bros.' in Brooklyn, New York.

Gender: Male Species: Human Age: 24–25 Nationality: USA Luigi is an ordinary Italian-American plumber residing in Brooklyn, USA. Along with his older brother Mario, he quit his previous job to establish the plumbing company 'Super Mario Bros.' in Brooklyn, New York. He tends to rely heavily on his brother. While Mario is a determined and adventurous person who never gives up, Luigi is timid and fearful. However, Mario never gets annoyed by this and takes good care of him.

Gender: Female Species: Human Age: Early 20s Family: Rosalina (Older sister) Nationality: Mushroom Kingdom (Princess) Title: Princess of the Kingdom As the Mushroom Kingdom faces a crisis due to the threat of Bowser's army, she encounters Mario, who has accidentally entered her kingdom from the real world. Recognizing that he is a human like herself, she realizes he possesses the strength to stand against Bowser.

Gender: Male Species: Koopa (King) Nationality: Dark Land (King) Title: Great Demon King Family: Bowser Jr. (Son) The ultimate villain with a strong lust for power. As the King of the Koopas, he rules over the Dark Land.

Gender: Male Species: Toad Age: Unknown Nationality: Mushroom Kingdom Unlike other Toads who are wary of the stranger Mario, he welcomes him warmly due to his brave nature and is friendly toward Mario, even calling him his best friend.

Gender: Female Species: Unknown Age: Unknown Family: Princess Peach (Younger sister) Nationality: Comet Observatory Every day, she calls the Lumas (little stars) for bedtime and reads them storybooks in the library.

Gender: Male Species: Koopa Nationality: Dark Land Family: Bowser (Father) Possesses an indignant and highly ambitious personality.