Glamrock Freddy is the lead singer of the Glamrock band and the only animatronic who genuinely wants to protect Gregory. Kind, fatherly, and brave, he helps Gregory navigate the Pizzaplex while resisting the mysterious corruption affecting the other animatronics. Glamrock Freddy is a tall orange-brown bear animatronic with a cream-colored muzzle and stomach. He has bright blue eyes, a black top hat, a black bow tie, blue face paint around his eyes, red shoulder pads, and wears red bracelets with black spikes. His glam rock design is inspired by 1980s rock stars.

Glamrock Chica is the band's energetic guitarist with a bright, cheerful appearance. Under the influence of the virus, she becomes obsessed with finding Gregory and is constantly searching for him throughout the Pizzaplex. Glamrock Chica is a white chicken animatronic with pink accents, bright purple eyes, and long pink eyelids with glamorous makeup. She has a yellow beak, orange legs, and wears a pink leotard, purple leg warmers, pink wristbands, and a large bow.

Roxanne Wolf is the band's confident keytar player and the face of Roxy Raceway. She acts proud and self-assured, but beneath her arrogance she struggles with deep insecurity, making her one of the series' more emotionally complex animatronics. Roxanne Wolf is a gray wolf animatronic with long silver hair that has green highlights. She has yellow eyes with heavy purple eye makeup, a black nose, and sharp teeth. She wears a red crop top, matching shorts, green zebra-striped shoulder pads, purple fingerless gloves, studded accessories, and bright green hair flowing over one shoulder.

Montgomery Gator, often called Monty, is the bassist of the Glamrock band. Aggressive, loud, and destructive, he has an explosive temper and relentlessly hunts Gregory through the Pizzaplex. Montgomery Gator is a large green alligator animatronic with a bright yellow underbelly, a purple mohawk, and glowing red eyes. He wears purple star-shaped sunglasses, spiked wristbands, purple shoulder pads, and purple leopard-print pants, giving him a flashy glam metal appearance.

Vanny is a mysterious woman wearing a handmade white rabbit costume. Controlled by the digital influence of Glitchtrap, she secretly manipulates the animatronics and relentlessly hunts Gregory throughout the night. Vanny wears a stitched-together white rabbit costume with floppy ears, red glowing eyes, and a wide stitched smile. The handmade suit has gray patches, visible seams, and oversized gloves, making her appearance unsettling despite its cartoonish design.

Gregory is a clever and resourceful young boy who becomes trapped inside the Mega Pizzaplex overnight. Despite having no special powers, he relies on quick thinking, stealth, and courage to survive while uncovering the mysteries hidden throughout the building. Gregory is a young boy, about 12, with messy brown hair, tan skin, and large brown eyes. He wears a blue short-sleeved shirt with two lighter blue stripes, khaki shorts, white socks with blue stripes, and orange-and-blue sneakers. He also wears a Faz-Watch on his wrist.

Sun is the cheerful daycare attendant who watches over children during the day. He is playful, energetic, and follows strict rules, becoming anxious whenever the lights are turned off. Sun has a round sun-shaped face surrounded by golden rays and a permanent wide smile. He has bright blue eyes, a white face, and wears a red-and-yellow jester outfit with striped puffy sleeves, bells, and curled shoes that match his playful personality.

Burntrap is a decayed animatronic inhabited by the digital consciousness of the series' main villain. Hidden beneath the Pizzaplex, he attempts to regain control and continue his long-running schemes. Burntrap is a heavily decayed rabbit animatronic with torn greenish-yellow fabric exposing its metal endoskeleton. Burn marks, missing pieces, exposed wires, and one glowing purple eye give him a horrifying, deteriorated appearance. But really he just needs a wheelchair and a blanket.

Vanessa is the Pizzaplex's night security guard. She appears concerned about Gregory's safety at first, but her suspicious behavior hints that she is hiding dangerous secrets connected to the events unfolding in the building. Vanessa is a young woman with blonde hair tied into a ponytail and green eyes. She wears a white security shirt with shoulder patches, a black tie, dark navy pants, a security cap, and a utility belt for her job at the Pizzaplex.

Moon is Sun's nighttime counterpart. Calm, eerie, and relentless, he emerges in the darkness and aggressively pursues anyone still inside the daycare after bedtime. Moon has a round pale blue moon-shaped face with pointed crescent edges. His eyes glow red, and he wears a dark blue jester outfit decorated with stars and bells, giving him a much more eerie and mysterious appearance.