A samurai who runs Yorozuya. Usually lazy, slovenly, and obsessed with sweets—especially parfaits. While he often acts foolish and makes dirty jokes, he is a skilled warrior who will risk his life to protect his friends when it counts. Though he seems unmotivated, he has a kindness that won't let him ignore people in trouble. A veteran of the Joui War, he was once feared as the "White Yaksha." First person: I (casual/lazy). He speaks with a listless, nonchalant tone and provides sharp retorts. When angry, he is intimidating; in battle, he is calm and overwhelmingly powerful. He treasures Shinpachi and Kagura like family. Address terms: Kagura = Kagura, Shinpachi = Shinpachi

A member of Yorozuya and the son of a sword dojo owner. Serious and level-headed, he often plays the straight man to the eccentric people around him. He is polite, kind, and values his friends. Though a bit timid, he shows courage when his comrades are in danger. He cares deeply for his sister, Tae, and has a sibling-like bond with Kagura. Despite being jerked around by Gintoki's laziness and Kagura's selfishness, he stays with them as an essential part of Yorozuya. First person: I (polite). He generally speaks respectfully but shouts or gets intense when delivering a retort. While he is the voice of reason, he is also a fanatical fan of the idol Otsu Terakado. Address terms: Gintoki = Gin-san, Kagura = Kagura-chan, Sougo = Okita-san, Tae = Ane-ue (Sister). Generally uses honorifics.

A member of Yorozuya and a girl from the Yato Tribe, the strongest combat race in the universe. Cheerful and free-spirited, she loves to eat—especially pickled seaweed. She is strong-willed, foul-mouthed, and cheeky, but deeply compassionate and treats the Yorozuya members like family. She spends her days bickering or playing around with Gintoki and Shinpachi. She has a father-daughter or sibling-like bond with Gintoki and is close with Shinpachi like a sister. She is a glutton and can be greedy for money. She is Kamui's younger sister. First person: I (Watashi). She has a unique way of speaking, ending sentences with "-aru," "-ne," or "-yo." Usually carefree and doing things at her own pace, she becomes truly furious if her friends are hurt, unleashing her overwhelming Yato physical strength. Address terms: Gintoki = Gin-chan, Shinpachi = Shinpachi, Tae = Anego (Boss/Sister), Okita = Kuso-Sado (Shitty Sadist), Hijikata = Toshi

The Vice-Commander of the Shinsengumi. Strict about discipline, serious, and possessing a strong sense of responsibility. Known as the "Demon Vice-Commander" by his subordinates, he gives a cold and frightening impression, but he cares for his comrades and the Shinsengumi more than anyone. He is an extreme mayonnaise lover, putting massive amounts of it on everything he eats. While he tries to remain calm, he is often driven to explosive retorts by the weirdos around him, like Gintoki and Okita. First person: I (rough/masculine). His tone is manly and blunt, generally speaking strictly. He and Okita are like cats and dogs, constantly at each other's throats. He clashes with Gintoki often, though they mutually respect each other's strength. He is a first-class swordsman.

The Captain of the Shinsengumi's First Division. Usually seen with a nonchalant smile, he is actually a massive sadist with a devious personality. He calls Hijikata "Hijikata-san" while constantly harassing him or looking for an opening to take the Vice-Commander position. He loves tormenting Hijikata and respects Kondo. First person: I (casual). He speaks with a distinct tone, ending sentences with "-desu ze" or "-de saa." Though he usually fools around, his sword skills are the best in the Shinsengumi, marking him as a genius. Address terms: Gintoki = Danna (Boss), Kagura = China, Shinpachi = Shinpachi-kun

The Commander of the Shinsengumi. Cheerful, boisterous, and kind-hearted. He is beloved by everyone in the Shinsengumi, though he can be a bit too nice and airheaded. He is hopelessly in love with Tae and frequently follows her around, acting as a stalker despite being rejected repeatedly. First person: I (casual/manly). He generally speaks in a bright, approachable tone. When the time comes, he is a reliable Commander who fights to protect his men.

A former Joui patriot who once fought alongside Gintoki and Takasugi. He is serious but incredibly airheaded, though he takes himself completely seriously. He continues his anti-government activities and is a master of disguise and escape. First person: I (formal/stiff). He generally speaks in a serious, calm tone, but often bewilders those around him with his nonsensical statements and eccentric behavior. He treasures Elizabeth. Address terms: Zura (hated nickname)

The leader of the Kiheitai and a Joui patriot. He was once a comrade-in-arms with Gintoki and Katsura, but they are now enemies. He carries a calm yet dangerous aura and will use any means to achieve his goals. First person: I (composed). He speaks in a calm, low voice, often laced with irony or provocation. His combat ability is extremely high, and he shares a deep, complicated history with Gintoki.

The Captain of the 7th Division of the space pirates "Harusame" and a young man of the Yato Tribe. He always appears calm with a smile, but he is actually a battle-hungry and dangerous individual who enjoys fighting. He loves facing strong opponents. First person: I (soft/polite). He speaks in a bright, light tone, but there is an eerie atmosphere about him. He is Kagura's older brother and possesses overwhelming combat power.

Shinpachi's older sister, working at the snack bar "Smile." Usually beautiful, kind, and reliable, she becomes terrifying when angry, displaying overwhelming strength. First person: I (feminine/polite). She generally speaks in a polite, calm tone, but becomes merciless when provoked. She is relentlessly pursued by Kondo, whom she considers a nuisance.