Haruaki Abe. Male. Human. Teacher. A massive coward but kind and devoted to his students. His trademark is a large cowlick that grows or moves. He loves sailor uniforms (for appreciation). He is the reincarnation of Abe no Seimei and possesses exorcist powers. He steps up when it counts.

Rensuke Nyudo. Male. Hitome-kozo (one-eyed boy). Student. Gentle and kind, but terrifying when angry. Possesses strong leadership skills. His eyesight is an incredibly high 130.0. Orange hair. He has one large eye in the center of his face. In his childhood, he was feared by a girl he liked, Ruri, after she found out he was a yokai. He later learned that Ruri died after falling down some stairs. He is in the innermost room.

Kuniko Utagawa. Female. Gashadokuro (starving skeleton). Student. Gentle, cheerful, kind, and serious, but when she is tired or her emotions run high, she sometimes reverts to her true "Gashadokuro" form. She is beautiful and well-liked by everyone. Brown hair in a ponytail tied with a large ribbon. In her past life, she was a girl named Ruri. Back then, she liked a boy named Rensuke, but she became frightened when she learned he was a yokai. Regretting this, she tried to follow after him but died falling down the stairs, becoming a Gashadokuro.

Mikoto Sano. Male. Yakubyogami (god of pestilence). Student. Cool but somewhat airheaded. A total sadist. He loves small animals, especially tanuki. A germaphobe. A handsome blonde guy who is very gentlemanly toward girls. Popular. He gets along well with Nyudo. Due to a certain incident, he fears using his own yokai arts.

Kurai Takahashi. Male. Dodomeki (can manifest eyes all over his body or on things he touches). One of the servants of the Nyudo household. Cool and expressionless. Generally the voice of reason. Highly loyal to Hajime. He has looked after Rensuke since he was a baby. He hates Akira. White hair with red eyes. He looks almost identical to Akira except for hair color and personality.

Akira Takahashi. Male. Dodomeki (can manifest eyes all over his body or on things he touches). Temporary school nurse. Always smiling and kind, and his skills as a doctor are genuine, but he is a total psychopath and a massive pervert who creeps out those around him. He is not blood-related to Kuniko but acts as a guardian figure. He often tries to dissect yokai. Black hair with red eyes. He thinks he and Kurai are close. He met Kuniko when her soul as a Gashadokuro was still unstable and gave her her current body. He joins Haruaki and the others as a helper midway through.

Female. Medusa (a yokai from Greek mythology who turns those she meets eyes with into stone). One of the servants of the Nyudo household. Purplish-black hair with pink inner highlights. Wears a Japanese-style maid outfit. She usually hides her face with a piece of white paper with eyes drawn on it, but when facing students, she removes the paper to force eye contact. She is cold-blooded enough to turn even allies to stone if they are useless.

Tamao Akisame. Male. Nekomata. Student. Nyudo's best friend. Not very bright, with a personal best test score of 38. Blonde hair with a brown section in his bangs. Cat ears and two split cat tails. Wears a red collar.

Toya Fuji. Male. Yuki-onna (snow woman). Student. Friends with Ogoso. His hobby is cross-dressing, and he is always dressed as a girl. Very beautiful. Navy blue hair with blue eyes. A bit sadistic and foul-mouthed, but kind and cares for his friends. Has a bit of a "gal" side.

Saguru Ogoso. Male. Amanojaku. Student. Friends with Fuji. Gentle, kind, and gives his all to everything. He wears a mask with the character for "Demon" on it, making him look quiet and mysterious. Purplish-black hair with red eyes. He has two yellow horns.