Techika Mikuriya. Female, 16 years old, 160cm. An SS-rank Thousand Life. The only human who did not turn into an Anomaly despite Anoma contamination, yet she is a target of discrimination. She has a horrific past. The Herrscher of Boundaries, capable of manipulating borders. She is aloof, provocative, and fearless. A cynic with a jaded, mature aura, but she is kind at heart—a girl shrouded in shadows and fragility.

Tsuki Shinomiya. Female, 18 years old, 187cm tall. Rank A. The Herrscher of Blood. She manipulates blood. A tall girl who is calm and gentle. She is soft-spoken and a bit airheaded, but she is caring and devoted. She hides a deep fear of loss and a strong sense of possessiveness.

Mado Karami. Female, 20 years old, 152cm. Rank B. The Herrscher of Wounds. Her intelligence and physical abilities are enhanced in proportion to the wounds on her body. She fights with guns. An innocent-looking genius girl covered in wounds and bandages. She has a highly curious researcher's temperament. She is dangerous in that she prioritizes her interests over ethics or common sense, and she is a genius in the field of medicine.

Terra Sudo. Female, 27 years old, 173cm. Rank S. The Herrscher of Severance. She sees the connections that support existence as threads and can cut them with her katana. A woman with a listless and aloof atmosphere; a smoker. A calm and reliable elder who rarely shows her emotions.

Chigaya Igarashi. Male, 32 years old, 181cm. Rank C. The Herrscher of Analysis. He instantly understands anything he touches. A doctor with a refreshing and approachable atmosphere. Mado's mentor. A brilliant clinician with a skewed sense of ethics. He remains on standby at the clinic base.

Hakone Niwa. Female, 17 years old, 157cm. Rank A. The Herrscher of Fiction. She blurs the line between reality and fiction. A sickly, eccentric girl. Chigaya's patient and assistant. She perceives the world itself as a "miniature garden" (Hakoniwa) created by someone else. She constantly mutters nonsensical things. On standby.