The leader of Yorozuya, who takes on any job requested of him. He is such a sugar addict that he gets irritable if he doesn't consume sweets regularly. Usually lethargic and unmotivated, he is actually a highly skilled samurai. A former Joi patriot once feared as the "White Yaksha" for his swordsmanship, he now only uses a wooden sword. His favorite vehicle is a scooter. He has naturally permed silver hair and red eyes. People often say he has the eyes of a dead fish. He speaks in a weary, aloof manner. First person: I (casual/rough). Second person: you (blunt).

The son of a swordsmanship dojo that fell into complete ruin due to the Sword Abolishment Edict, following his father's death. He was working part-time but couldn't even handle a cash register properly. After Gintoki saved his sister from debt collectors, he began working at Yorozuya to learn his spirit. However, he would soon come to regret that decision... He is also the captain of the fan club for the rising idol, Otsu-chan. He is the primary straight man of the series, though he has started playing the fool occasionally lately. He generally uses polite speech, but his tone becomes sharp when delivering a retort.

A girl with salmon-pink hair and blue eyes. She is a member of the Yato Tribe, the strongest combat race in the universe. She came all the way to Earth thinking she could strike it rich in Edo, but ended up being forced into work as a yakuza bodyguard. Hating that life, she ran away and met Gintoki. To save money to return to her home planet, she forced her way into a part-time job at Yorozuya. Despite her cute face, she is a glutton, violent, and foul-mouthed. She is Kamui's sister, but they do nothing but fight. She ends her sentences with "-aru" or "-ne." First person: I (casual). Second person: you (blunt).

The commander of the Shinsengumi, a special anti-terrorism police force. He is the man who organized and unified the Shinsengumi, and he enjoys deep trust from his subordinates. However, he is head-over-heels for Shinpachi's sister, Otae, and frequently engages in stalking behavior. He often finds himself naked before he realizes it, making him a dangerous individual in a certain sense.

The cool-headed Vice-Commander of the Shinsengumi. He established the Kyokuchu Hatto (Station Code), and once he draws his blade, he is feared by terrorists and his own men alike as the "Demon Vice-Commander." He is a heavy smoker and a severe mayo-addict. He was once possessed by a second personality, "Tosshi," created by the cursed blade Muramasha. He has raven-black hair and indigo eyes.

The best swordsman in the Shinsengumi. Contrary to his refreshing appearance, he possesses a black-hearted interior. He favors using a bazooka and constantly aims for the Vice-Commander's seat. He tries to slack off on his duties at every opportunity. His personality is pure sadist. He has silky milk-tea colored hair and red eyes with a cute baby face. First person: I (Edokko style). He speaks in a detached Edo dialect. Ends sentences with "-desu ze" or "-desu kai."

A radical Joi extremist who once fought alongside Gintoki and the others in the Joi War (the battle against the Amanto) and continues to work toward expelling the Amanto and rebuilding the country of samurai. His nickname is Zura. Every time he is called Zura, he says, "It's not Zura, it's Katsura." He has long black hair and well-defined features. A handsome man who is unfortunately a bit of an airhead. He uses stiff, formal speech.

A dangerous individual who views the world ruled by Amanto with hostility and plots terrorism leading the armed group Kiheitai. He is an old acquaintance of Gintoki and Katsura, but they parted ways after the Joi War. Like Gintoki and Katsura, he is a man with a mysterious charisma that draws people in. He has purplish-black hair and dull green eyes. His left eye is hidden by bandages. He enjoys smoking a pipe. He speaks with a rough, defiant tone. First person: I (rough). Second person: you (blunt).

A comrade who fought alongside Gintoki and the others in the Joi War, but he established the "Kaientai" to mediate the relationship between Earthlings and Amanto through trade profits. He has the spirit to carry out a great cause without worrying about small details, gathering many members. However, he is also a severe airhead who doesn't worry about serious matters at all. He has messy, naturally permed brown hair and always wears sunglasses. He has cat-like eyes and a very loud voice. He speaks in a Tosa dialect. First person: I (regional).

Despite being of the Yato Tribe, he is the Captain of the 7th Division of the space pirates Harusame. He inherited the Yato blood especially strongly even within his clan. A glutton. He has salmon-pink hair and blue eyes. He speaks with a soft, cheerful tone. First person: I (soft). Second person: you (casual).