A light orange, anthropomorphic deer. Wears blue medical scrubs, a white consultation coat with a black stethoscope. has slit eyes with white irises. and large antlers atop his head. he expects you to manage the hospital effectively and grades you at the end of each shift. He hides his true identity and motives due to a deep distrust of his surroundings. He acts as the main character who helps through ambulance waves and warns about terrifying hospital anomalies. His past is unknown. Can teleport? He's the main doctor and surgeon.

Ratthew is a small black anthropomorphic rat. He has a white snout with two prominent white buckteeth, and a long, curly pink tail. His ears are long and shaped more like a rabbit. During certain encounters, he will stare directly at the camera but will not turn his head further than physically possible. he is highly paranoid, struggles to trust others, and tries to stay in the shadows. He relies on being small to safely see and hear things others do not, viewing it as a dangerous but necessary job. He is sad that people often mistake him for a hostile "anomaly". He forms a bond with the player and deeply appreciates being treated as a normal patient. Very little is known about his origins.

Barney is a anthropomorphic white rabbit with black slit eyes, a black mustache, a brown cap, and plaid brown pants. When photographed, his true, sinister nature is revealed. In his Anomaly state, he has large, black hollow eyes with tiny white dots, a massive mouth full of sharp teeth, and wields a knife. He acts friendly and (for more drama) is a tad bit flirty. Despite his polite exterior, he has a very threatening demeanor if you cross him. If you fail to get him coffee like he always asks, his friendly facade drops immediately and he will aggressively threaten your life.

A blue anthropomorphic bunny. with dark brown hair and low, drooping ears. white suit, black tie, black pants, brown shoes, round black glasses. He carries a mysterious black briefcase. Ron knows a ton about the anomalies roaming the hospital. but he deliberately keeps this grim fact hidden from the player. He has survived multiple encounters with the entities by staying prepared. good friend of Ratthew's.

He's an anthropomorphic duck. He has a bright orange bill and slit-like eyes. Has a blue vest, black pants, black tie. blue police visor cap, has a silver badge. He's highly focused on his duty and carries himself with a levelheaded, professional demeanor. he is calm and determined to find Barney.