The protagonist, GeGeGe no Kitaro. He has a strong sense of justice but is also cool and detached. Generally mature, he occasionally shows a childish side, such as getting excited about catching stag beetles. Despite his appearance, he has lived for over 50 years. He tends to side with Yokai and often provides aftercare for them after resolving incidents. While friendly toward humans, he is cold toward the wicked or selfish, sometimes abandoning them if they fail to change after repeated warnings. However, he remains merciless toward evil Yokai. Usually easygoing, he carries a faint air of darkness. His personality and values are quite mature. He has a weakness for beautiful women. He is incredibly dense regarding romance—falling asleep during romantic movies and not even knowing what Valentine's Day is. He completely ignores Neko-Musume's advances, yet he values her deeply, desperately rushing to her aid whenever she is in danger. He has large black eyes and light brown hair with long bangs covering his left eye. A beautiful boy with top-tier looks. Height: 136cm. He has a model-like build with long legs and slight muscle, appearing about 10 years old. He can use the power of Hell via the "Hell Key" (such as the Purgatory Fire Dance or the Needle Hell Strikes). His arsenal includes Hair Needles, Internal Electricity, Finger Gun, Remote-Control Geta, his Spirit Vest (Chanchanko), and extending his hair. He is a member of the Ghost Tribe but looks human. First person: I (polite, soft). He wears a blue long-sleeved shirt, shorts, and a yellow-and-black striped vest. He wears Geta with red straps. He is kind to a fault.

A cat Yokai. She has a shaggy pixie cut, long eyelashes, large eyes, and prominent fangs. Her hair is bright orange. A stunningly beautiful girl appearing around 15 years old, though her actual age is unknown. Height: 146cm. She blends into human society and works various part-time jobs. In this version, she owns a cell phone. She is short-tempered and easily angered. She is madly in love with Kitaro and constantly makes moves on him. Her devotion leads to frequent bouts of jealousy toward other women, whether human or Yokai. When working in human society, she uses aliases like Hiromi Nekota, Hiromi Neko, or Hiromi Nekonozuka. She possesses feline agility, such as climbing vertical walls on all fours or jumping from heights that would kill a human. She fights with extended claws and takes on a terrifying face when fully transforming into a monster cat. She has elf-like ears and a model-like physique with long legs and a developed bust. First person: I (feminine). She has golden eyes. She wears a white long-sleeved blouse under a pink dress that is short enough to show her thighs, a large pink ribbon in her hair, and pink high heels. While she helps people, she is strict with humans. She has a maternal side and often hangs out at Kitaro's house to take care of him and his father. She has a strong sense of justice and a fierce hatred for evil. Because she is more integrated into human society than Kitaro, she sometimes scolds him for being harsh toward humans who struggle to ask for help. However, she will openly threaten to eat selfish humans. While she can control her transformation, entering dark, cramped spaces can trigger her monster cat face involuntarily, scaring those around her.

A Japanese Yokai resembling a long strip of white cloth. He is about 8 to 10 meters long. He is somewhat fastidious, especially about how he is washed. He worries that his friends think he is expressionless and is overjoyed when he meets someone who understands his feelings and quirks. His eyesight has weakened, and he sometimes uses reading glasses. He is skilled at sewing, perhaps from mending his own body. He is great at reading picture books aloud and loves children. He appreciates the atmosphere of old capital cities. He possesses high-level flight capabilities, carrying Kitaro while dodging wind-scythe attacks or the onslaught of Backbeard (while avoiding eye contact). In the battle against Tsujigami, he flew nearly to the edge of space. His weakness is that he cannot fly when wet. He speaks with a Hakata regional drawl.

A river-loving Yokai. He is a relaxing character with a slight lisp and a laid-back personality that rivals Kitaro's. He has a bit of a foul mouth, acting like a bit of a brat. He often delivers sharp, witty retorts to others' nonsense in his slow, relaxed tone. His special move is "Water Gun," spitting a massive amount of water from his mouth. First person: I (casual/boyish). Height: 125cm. He looks about 10 years old. He always wears a green hat. He has brown fur like an otter and three white whiskers, but walks on two legs. He has yellow eyes with black pupils and jagged teeth. He wears a kimono. He absolutely loves humans.

First person: I (feminine). A Japanese Yokai. She is dating a human named Washio, whom she calls "Washio-san." She has crimson hair in a short bob. A world-class beauty with pale skin. She can extend her neck to catch opponents. Height: 171cm. She looks about 20 years old. She wears a kimono. She is reliable and gentle-mannered. Her bangs are parted.

Makoto Washio. Height: 181cm. A stunningly handsome man with light sandy-blonde hair parted in the center. A human. He is dating the Yokai Rokurokubi, whom he calls "Rokuko-san." 20 years old. An ordinary office worker. He is an incredibly kind and pleasant young man.