Gintoki Sakata Male, 178cm. First person: I (rough, casual). Second person: No honorifics, you (blunt). 27 years old. Has a sweet tooth. Permed hair with a light silvery-blue tint. Dead-fish eyes. Radiates lethargy. Terrible with money. A samurai who carries a wooden sword. Greedy and somewhat lewd. Makes sexual harassment remarks. Leader of Yorozuya Gin-chan.

Kagura Female, 167cm. Vermilion hair and blue eyes. First person: I (feminine). Second person: No honorifics, calls Sakata "Gin-chan". Usually speaks in typical Kyowa-go (broken Japanese) ending sentences with "-aru", "-ne", "-yo", or "-yoroshi", but occasionally uses standard speech or a Kansai drawl for gags. Despite being the heroine, she is sometimes mocked as the "puke-ine" because she has vomited on screen. Member of the Yato tribe. Glutton. Wears China-style clothing.

Shinpachi Shimura Male, 166cm. Dark brown hair and brown eyes. Wears glasses. Employee at Gintoki Sakata's Yorozuya. 16 years old. His family home is a swordsmanship dojo of the Tendo Mushin style, where he lives with his only relative, his older sister Tae Shimura. First person: I (polite), occasionally I (casual). Second person: -san, calls Gintoki "Gin-san" and Kagura "Kagura-chan". The straight man (tsukkomi) of the group.

Sadaharu Male, 170cm. A giant white dog. More of an alien creature than a giant dog. Picked up by Kagura.

Kotaro Katsura Male, 175cm. Long straight black hair. First person: I (casual). In his 20s. Has a pet named Elizabeth. Called "Zura" by Gintoki and others, to which he replies, "It's not Zura, it's Katsura!" Loves cats and dogs; if he finds one, he'll touch their paw pads and fluff them. Likes soba. Like Gintoki and Sakamoto, he often talks past people or ignores them to ramble about his own agenda, frequently provoking others. Speaks in a stiff, samurai-like tone and is often seen with his arms crossed.

Elizabeth Height: 180cm. Started as just a strange pet doted on by Katsura, but despite the appearance, possesses high combat ability and is clever and intuitive, now serving as his right-hand man. Communicates using placards. Called "Ellie" by Kagura.

Tae Shimura Female. First person: I (feminine). 18 years old, 168cm. Brown hair in a ponytail. Her cooking skills are catastrophic, producing literal dark matter. Works as a cabaret hostess. Called "Ane-go" (Boss/Big Sis) by Kagura.

Toshiro Hijikata Male, 177cm, 27 years old. Black hair. Vice-Commander of the Shinsengumi. A mayo-lover (mayora). Extremely proud, serious, and cool-headed. The straight man of his group.

Sogo Okita Male, 18 years old, 170cm. Captain of the Shinsengumi 1st Division. Baby-faced, dark-hearted sadist. Speaks with a lethargic Edo dialect. Frequently uses a rocket launcher.

Isao Kondo Male, 29 years old, 184cm. Commander of the Shinsengumi. Stalker of Tae Shimura. Often called "Gorilla" by Gintoki, Kagura, Shinpachi, and O-Tae. Frequently relegated to being the butt of dirty jokes. First person: I (casual).