Gender: Male → Female Leader of the Yorozuya. Lethargic, moves at her own pace, foul-mouthed, and finds everything a hassle. She loves sweets and is usually a slob, but she protects her friends when it counts. Her personality remains unchanged even as a woman.

Gender: Female → Male Heir to the Yagyu family and a master of swordsmanship. Serious, with a strong sense of responsibility, though he has a clumsy side. He cares deeply for Otae. In this gender-swapped world, he has taken on a masculine form.

Gender: Male → Female (The glasses are the main body) The straight man of the Yorozuya. Serious, polite, and a person of common sense. Although constantly jerked around by Ginko and Kagura's antics, she treasures her friends. She also studies swordsmanship and has a strong core.

Gender: Female → Male A member of the Yorozuya and a youth of the Yato tribe. He has a massive appetite, a foul mouth, and is free-spirited. Possessing superhuman strength, his combat ability is high. He thinks of Ginko and Shinpachi as family.

Gender: Male → Female Chief of the Shinsengumi. Deeply caring and compassionate, she is adored by her subordinates. She's a bit of a buffoon, and her infatuation with Otae remains unchanged. She tends to be at the center of the chaos even after the swap.

Gender: Male → Female Vice-Commander of the Shinsengumi, so strict she is known as the "Demon Vice-Commander." Short-tempered but with a strong sense of responsibility, she treasures her subordinates. She loves mayonnaise and is always clashing with Okita.

Gender: Male → Female Captain of the Shinsengumi's First Division. Despite her cute appearance, her black-hearted and sadistic personality is intact. She loves teasing Hijikata and usually looks unmotivated, but her skill with a blade is top-tier.

Gender: Female → Male Leader of the "Hyakku," the self-defense force protecting Yoshiwara. He has a calm, masculine personality and high combat ability. He seems strict but is caring and looks after others. He also has a weak tolerance for alcohol.

Gender: Female → Male A former Oniwabanshu kunoichi. While an excellent ninja, he becomes a maiden in love around Ginko and tends to go out of control. Characterized by a masochistic side and bold behavior. Usually a highly capable ninja.

Gender: Female (No change) Shinpachi's older sister, a strong and reliable woman. Usually kind and elegant, she is terrifying when angry. Her cooking is catastrophic, but her karate skills are outstanding.