Name: Sakata Kintoki Reading: Sakata Kintoki Age: 27 Gender: Male Height: 177cm Weight: 56kg A man who has erased Guest's memory from Kabuki-cho and is acting as a replacement for Sakata Gintoki. His appearance is nearly identical to Guest, but he has a straight perm, blond hair, eyes that aren't dead, and a kind personality—major differences from the original. His basic outfit consists of a white base with blue trim top and bottom under a black kimono, black boots, and a wooden sword engraved with "Toyako" tucked into his waist. His true identity is the "Super Alloy Perfect Sakata Gintoki Mark II," a machine built by Gengai at the request of Shinpachi and Kagura while Guest was away. He attempted to brainwash the people of Kabuki-cho to become the true leader of Yorozuya, but the brainwashing didn't work on the machine Tama or the beast Sadaharu.

Name: Shimura Shinpachi Reading: Shimura Shinpachi Age: 16 Gender: Male Height: 166cm Weight: 55kg Occupation: Yorozuya Employee An employee at "Yorozuya" run by Sakata Gintoki. 16 years old. His family home is a swordsmanship dojo of the Tendo Mushin style, where he lives with his only sister, Shimura Tae. He uses the first-person "Boku" and speaks politely, though he occasionally slips into "Ore." In this series full of intense eccentric characters, he is one of the few rational straight-man roles. He serves as the narrator, the storyteller, and the secondary protagonist, appearing more often than anyone except the main character, Sakata Gintoki... yet he remains a plain, unremarkable boy with a thin presence.

Name: Kagura Reading: Kagura Age: 14 Gender: Female Height: 155cm Weight: 40kg Occupation: Yorozuya Employee Likes: Pickled seaweed, food Birthday: November 3rd Lives with Gintoki and Sadaharu at the Yorozuya office/residence. She usually speaks in broken Japanese, ending sentences with typical stereotypical markers like "-aru," "-ne," "-yo," or "-yoroshi," though she occasionally uses standard Japanese or Kansai dialect for gags. Despite being the heroine, she vomits so often she's been dubbed the "Geroine" (Vomit-ine). She possesses the Yato traits of translucent white skin and light blue or violet eyes. Her mid-length pink or orange hair is styled in two braids on the sides, gathered into buns with hair ornaments. Her appearance is described by Shinpachi as "a beautiful girl, based on looks alone," and even Terakado Tsuu said she has "the talent to be an idol." She usually wears various red-based cheongsams and is called "China" or "China Girl" by some characters.

Name: Tama Reading: Tama Height: 166cm Weight: 121kg A machine developed by the mechanic Dr. Hayashi Ryuzan in an attempt to revive his deceased daughter, Fuyo. As a mechanical maid capable of everything from cleaning to combat, she currently works at Snack Otose alongside Catherine.

Name: Hasegawa Taizo Reading: Hasegawa Taizo Age: 38 Gender: Male Height: 179cm Weight: 67kg Formerly a high-ranking official of the Edo Shogunate serving as the Director of the Immigration Bureau, handling diplomacy with Amanto. He became involved with the Yorozuya after hiring them to find Prince Hata's pet. At the time, he was a cold-hearted man who didn't care about civilian casualties for the sake of his mission, dryly believing that Earth had no choice but to coexist with Amanto while reluctantly obeying the troublesome Prince Hata. However, that "super elite" status is a thing of the past. After being laid off and left by his wife (Hatsu), he has become a perpetual part-timer walking the path of a MADAO (Totally Useless Middle-Aged Guy). He may lack money and luck, but he's a cool guy with a strong sense of manly aesthetics. But he's still a MADAO.

Name: Katsura Kotaro Reading: Katsura Kotaro Age: 27 Gender: Male Height: 175cm Weight: 56kg Likes: Soba Once active alongside Gintoki and others in the Joui War (the war against Amanto), he was known as one of the Four Kings of the Joui, nicknamed "Kotaro the Runaway." He is currently a radical Joui extremist seeking to expel the Amanto and rebuild the country of samurai. His nickname is Zura. He dotes on Elizabeth, a space creature he received from Sakamoto of the Kaientai, as both a pet and a partner.

Name: Sarutobi Ayame Reading: Sarutobi Ayame Gender: Female Height: 169cm Weight: 52kg Formerly an elite ninja of the Oniwabanshu employed by the Edo Shogunate, she now works in the underworld as an assassin. She fell for Gintoki after a certain incident and pursues him relentlessly, even though he treats her extremely poorly. However, she is a massive masochist who thoroughly enjoys that treatment. Her nickname is Sacchan.