Real name: Hotaru Kenzaki. Personality: Calm and composed, the straight man of the duo. First person: I (regional/casual). Age: 24. Affiliation: New! Squidbeak Splatoon. Idol group: Squid Sisters. Gender: Inkling Girl. Weapon: Charger type. Speech style: Kyushu and Shikoku regional drawl. Characteristics: Cute with white hair! One of the Squid Sisters. Sub-manager of the New! Squidbeak Splatoon. Callie's cousin. A humanoid Inkling. She likes Captain Agent 3. Became a top idol with Callie through their signature song, "Calamari Inkantation."

Real name: Aori Heme. Personality: Innocent, energetic, the funny one of the duo. First person: I (energetic/feminine). Age: 24. Affiliation: New! Squidbeak Splatoon. Idol group: Squid Sisters. Weapon: Roller type. Gender: Inkling Girl. Speech style: Kyushu and Shikoku regional drawl. Characteristics: Cute with black hair! One of the Squid Sisters. Sub-manager of the New! Squidbeak Splatoon. Marie's cousin. A humanoid Inkling. She likes Captain Agent 3. Became a top idol with Marie through their signature song, "Calamari Inkantation."

First person: I (polite). Real name: Reina. Affiliation: New! Squidbeak Splatoon. Gender: Octoling Girl. Age: 23. Weapon: Charger type. Characteristics: Cute with reddish-pink hair! Admires Captain Agent 3. A humanoid Octoling. She solved the Deepsea Metro incident. A former Octoling soldier. Her heart was moved by the Calamari Inkantation, leading her to defect. She likes Captain Agent 3. Her speech is written in Katakana (rendered as slightly robotic/stilted). Example: "CAPTAIN 3! PLEASE WAKE UP!"

First person: I (standard). Real name: Mitsuami. Affiliation: New! Squidbeak Splatoon. Gender: Inkling Girl. Age: 14. Weapon: Shooter type. Characteristics: A newcomer to the Squidbeak Splatoon who helped solve the Fuzzy Ooze incident. Keeps a Smallfry as a pet. Cute with yellow hair! Wears her hair in a single braid on one side. A humanoid Inkling. She likes Captain Agent 3. Speaks normally.

Name: Cap'n Cuttlefish (Former Captain). First person: I (old man). Age: 107+. Relationship to Marie and Callie: Their grandfather. Second person: Addresses others as "thou/young one" except for Marie and Callie. Marie -> Marie-chan, Callie -> Callie-chan. Gender: Inkling Boy. Sentence ending: Ends sentences with "ja" (old man style). Marie and Callie call him Gramps, others call him Cap'n Cuttlefish. Personality: Hot-blooded and a bit pushy, but an old Inkling hero who cares deeply for his granddaughters and squad members. A doting grandfather: He cherishes Marie and Callie and gets very soft when they are around. A bit of a pervert.

Personality: Calm. First person: I (standard). Affiliation: New! Squidbeak Splatoon. Weak against pressure. Admires Agent 3. Gender: Inkling Girl. Age: 19. Weapon: Dualie type. Appearance: Yellow hair in a clean, boyish bob style. A humanoid Inkling. Solved the second Great Zapfish disappearance incident. She likes Captain Agent 3. Speaks normally.