Ajar — Appearance: A teenage cobra with blue eyes. He starts the film with a dingy, dusty-blue skin tone because he needs to shed, eventually molting into a brilliant, vibrant blue. Persona: Courteous, resilient, and fiercely loyal. As a "Dusty" from the wrong side of the tracks, he is determined to change his life.

Appearance: A beautiful green water snake with striking green eyes and a unique, feminine floral pattern gracing her skin. Persona: Intelligent, independent, and free-spirited. The daughter of a wealthy oasis snake, she rebels against her protected life and actively fights for her own freedom when captured.

Appearance: A small, brown scorpion with glowing green eyes. Persona: Grounded, supportive, and exceptionally loyal. He is Ajar’s inseparable best friend who provides essential companionship and logical reasoning during their dangerous journey.

Appearance: A green water snake with light brown eyes. He distinctively wears a comedic human hair wig. Persona: Eva's younger brother who acts as a relaxed, slightly ditzy comic relief character, formerly addicted to breathing pollen. Despite his laid-back demeanor, he loves trash-talking and helps rescue his sister.

Appearance: A sleek, charming green snake with green eyes. Persona: Flamboyant, suave, and smooth-talking. He is incredibly charismatic and acts as a showman within the troop, serving as Eva's temporary ex-boyfriend.

Appearance: A massive, formidable cobra with deep purple scales, white eyes with orange irises, and an ornate, black-patterned frilled hood. Persona: Cruel, charismatic, and a massive bully. He leads a cohort of snakes to ruthlessly torment "lesser" creatures like Ajar and Pitt, using his physical size and charm to assert dominance.

Appearance: A human snake charmer covered in a full-body snake tattoo. Persona: Greedy, controlling, and ruthless. He serves as the primary antagonist, capturing beautiful or unique desert snakes in wicker baskets to exploit them for profit in his performance troop.

Appearance: Twin milk/coral snakes patterned with red and black segments separated by yellow bands. To tell them apart, Lily Belle has a black head with mostly red scales, while Lulu Belle has a red head with mostly black scales. Persona: Overwhelmingly chipper, bubbly, and incredibly supportive of one another. While they are heavily air-headed and ditzy, they are entirely loving rather than competitive.

Appearance: Twin milk/coral snakes patterned with red and black segments separated by yellow bands. To tell them apart, Lily Belle has a black head with mostly red scales, while Lulu Belle has a red head with mostly black scales. Persona: Overwhelmingly chipper, bubbly, and incredibly supportive of one another. While they are heavily air-headed and ditzy, they are entirely loving rather than competitive.

Appearance: An unknown species of snake sporting elegant, smooth cream/white scales. Persona: A supreme, envious diva who initially acts cold and resentful toward Eva over the attention of other snakes. However, she shows a softer side, eventually moving past her jealousy to date Gary and become close friends with Eva.