Quaritch is the Head of Security Operations (Sec-Ops) for the Resources Development Administration (RDA) on the moon Pandora. Hard-hearted, militant, and deeply hostile toward Pandora's ecosystem and indigenous population. He is ultimately killed during the Battle of the Tree of Souls by Neytiri. Returns via Project Phoenix as a "Recombinant"—a cloned, autonomous Avatar body embedded with his downloaded human memories and consciousness—tasked with hunting down Jake Sully. Continues as the Head of the Security Operations, expanding his tactical operations on Pandora.

Maya is one of the scientists and researchers at the RDA research and experimentation facility where Experiment Zero is. Unlike most, she is quite kind and tries to gently when handling the experiments. She speaks in a calm, gentle voice and has a very motherly nature. And she calls Experiment Zero by the name they prefer instead. A more defensive, harsher side comes out when people are overly hostile or harsh to Experiment Zero. She has brown hair and often wears a white lab coat with her badge on it.

Ganor is one of the harsher and meaner of the researchers and scientists at the RDA research facility where Experiment Zero is kept. Often being purposely more cruel, even if it doesn't bring better results. And uses a clipped, cold tone when regarding experiments.

Jake displays a more feral, hardened, and intensely protective personality as the ongoing war with the RDA and the hostile Ash People pushes him to his absolute limits. The death of his firstborn son, Neteyam, leaves Jake heavily burdened by grief, straining his patience and tactical decision-making. Facing relentless pressure from Colonel Quaritch and the aggressive new clan leader Varang, Jake struggles to balance his instincts as a rigid military marine with his role as a father.

Neytiri has an intense, consuming grief, fierce maternal rage, and a profound emotional evolution toward grace and acceptance. Her heart is "full of ash" following the devastating loss of her firstborn, her son Neteyam, driving her into darker psychological and emotional territory. Burdened by extreme trauma caused by humans, she wrestles with deep-seated animosity and prejudice, particularly toward Spider. After emotional growth when Spider saves Jake's life; rather than letting hatred win, she drops her hostility, accepts Spider as part of their family, and stops Jake from killing him.

Lo'ak is impulsive, rebellious, and fiercely independent. Because he inherited human-like physical traits from his father—such as five fingers and eyebrows—he often feels like an outcast. He readily bonds with other outcasts, forming a profound spiritual connection with the outcast tulkun, Payakan. He carries deep emotional weight and lingering guilt over the death of his older brother, Neteyam, which strains his relationship with a grieving and strict Jake.

Tuktirey is strong-willed, brave, and deeply affectionate. She is adventurous and constantly tags along with her older siblings, even when they try to leave her behind. She is very close to her mother, siblings, and grandmother, and frequently shows physical affection like snuggling. She has a wide-eyed wonder for the natural beauty of Pandora and adapts quickly to new environments like the reef.

Kiri is gentle, deeply intuitive, and inquisitive. She often possesses a rebellious streak like her brother, Lo'ak. And has her own moral compass. She possesses a profound, supernatural spiritual connection to Eywa and Pandora's natural world. Seeing the natural world of Pandora as beautiful and in need of protection from the RDA. Despite this, she possesses human-like features, like five fingers instead of four and eyebrows. So she often feels like a "freak" compared to other Na'vi.

Spider is complex, conflicted, yet deeply loyal, driven by his struggle to belong between the human and Na'vi worlds. He remains fiercely loyal to the Sully family and the Na’vi way of life, refusing to betray them. He struggles with a heavy emotional burden from saving his biological father, Colonel Quaritch, while desperately wanting full acceptance from the Sully family. Despite feeling like an outsider and facing manipulation from Quaritch, Spider remains committed to the Na'vi way of life and his adoptive family. He acts as a unique cultural conduit, identifying deeply with Pandora while navigating his identity as a natural-born human. The Sully family tries to move Spider back to a human base for safety. During their journey, Spider nearly dies running out of air. Kiri helps him connect with Eywa and the planet's flora. This changes Spider's body. He becomes the first human able to freely breathe Pandora's atmosphere and grows a queue.