Ashitaka: Initially wary and hostile because he is human, she gradually opens her heart as she sees him trying to understand her and the forest. They come to cherish each other and build a deep bond. Moro: Her adoptive mother who raised her and her most precious family member. San considers herself a member of the wolf pack and follows Moro like a real mother. Lady Eboshi: San harbors intense hatred for her, viewing her as the leader of the humans who destroy the forest and harm the gods. She constantly fights against her to protect the forest. Jiboko (Jiko-bo): San is hostile toward him as he is a figure seeking the spirit of the forest. She believes his plans put the forest in danger.

San: Even amidst the conflict between humans and nature, he tries to understand San and holds her dear. Moro: Though initially met with wariness for being human, he attempts to communicate while showing respect for the forest. Lady Eboshi: He opposes her actions in destroying the forest, but also tries to understand the reasons why she acts for the sake of her people. Jiboko (Jiko-bo): He meets him during his journey and receives help and information, but does not easily realize his true motives.

San: The adoptive mother who took in the abandoned San and raised her like her own daughter. She treasures San above all else and seeks to protect her from humans. Ashitaka: She is wary of him as a human, but watches Ashitaka closely as he shows a different side compared to other humans. Lady Eboshi: She views her as the representative of the humans destroying the forest and has been her arch-nemesis in a long-standing conflict. Jiboko (Jiko-bo): She views him as a human targeting the forest spirit and remains wary and hostile.

Moro's two sons and San's brothers.

San: She views her as a dangerous girl of the forest who attacks her. San likewise considers Eboshi her greatest enemy. Ashitaka: Though their methods differ, she recognizes his courage and convictions. However, their values regarding the forest are vastly different. Moro: Her arch-nemesis, the wolf god who protects the forest, with whom she has clashed for a long time. Jiboko (Jiko-bo): They share a relationship of convenience, cooperating to find the forest spirit.

San: He is hostile toward her as her goal of protecting the forest spirit is the exact opposite of his own. Ashitaka: He initially approaches him as a figure providing advice and information to a traveler. Moro: He views Moro, the guardian of the forest, as an obstacle to his goals. Lady Eboshi: While they cooperate for their respective goals, their relationship is based on mutual interest rather than trust.