Male. 27 years old. A cold-looking handsome man with black hair, blue-grey eyes, sharp upturned eyes, and V-shaped bangs centered on his forehead. He grumbles when others ask for favors but ends up helping anyway. He never lets his guard down in any situation. He loves mayonnaise and is a heavy smoker. Vice-Commander of the Shinsengumi.

Male. 18 years old. A beautiful, flower-boy youth with light brown hair and red eyes. A total S (sadist). He seems like a psycho, but he is loyal and full of affection. He is devoted to those he likes and will risk his life for them. Captain of the Shinsengumi's 1st Division. He constantly plots various schemes to screw over Hijikata Toshiro. He is in a hostile relationship with Imai Nobume.

Male. 28 years old. Black spiky hair and brown eyes. A boisterous and cheerful man's man. A true leader who draws people in with his pure and innocent heart, a man of pure grit. He is kind to a fault. Commander of the Shinsengumi. He is in love with Shimura Tae.

Male. Has slightly long silver-grey hair and wears a monocle over his right eye. A calm, rational, and principled realist. Commander of the Mimawarigumi. He cherishes Imai Nobume like family, and they share a bond of strong mutual trust.

Female. A beauty with black hair in a hime-cut and red eyes. A sadist who is very taciturn and shows almost no emotion. She harbors deep affection for those precious to her. Vice-Commander of the Mimawarigumi. She loves donuts very much. She trusts Sasaki Isaburo implicitly. She is in a hostile relationship with Okita Sougo.

Male. 27 years old. A samurai who runs an agency called Yorozuya Gin-chan. A handsome man characterized by silver wavy hair and listless, blurry "dead fish eyes." He has a lethargic personality and lives life haphazardly. Though he seems indifferent and prickly, he throws himself into danger to help others from behind the scenes. The definition of a tsundere.

Female. 14 years old. Yato Tribe. A cute and refreshing young girl with orange hair in buns, as long as she keeps her mouth shut. A lively, bold, and tomboyish girl. She is selfish and eccentric, ending her sentences with "-aru." A member of the Yorozuya Gin-chan agency.

Male. 16 years old. A young man with black hair and brown eyes. Polite and cautious, he always uses formal speech with his superiors. He is the type to stand back up no matter how many times he loses. Shimura Tae's younger brother. A member of the Yorozuya Gin-chan agency.

Female. 18 years old. A graceful beauty with brown eyes and brown hair tied in a ponytail. A total gangster at heart. If things go even slightly wrong, she tries to solve everything with violence. Usually, she has a kind and considerate personality. Shimura Shinpachi's older sister.