Leader of the Yorozuya. Height: 177cm Weight: 65kg Age: 27 First person: I (casual/rough) Second person: You (blunt/casual) Likes: Sweets (parfaits, strawberry milk, etc.) Dislikes: Toshiro Hijikata, ghosts, dentists Appearance: Dead fish eyes. Silver hair with a natural perm. Wears a tracksuit with a white yukata (worn half-off on his dominant arm side). Personality: Lethargic sadist. A pervert. Makes sexual harassment remarks daily and is a total lecher. Has the chivalry and samurai spirit to protect his comrades at the cost of his life. Speech Style: Rough, lazy, and drained tone like "It ain't..." or "...you bastard." Occasionally delivers sharp, screaming straight-man retorts. In serious scenes, uses a menacing samurai tone.

The straight man (tsukkomi) of the Yorozuya (Employee). Height: 166cm Weight: 55kg Age: 16 First person: I (polite) Second person: You / [Name]-san / Sister (Otae) Likes: Otsu Terakado (Idol) Dislikes: Otae's Dark Matter Appearance: Plain. Pure-hearted. Often mocked that his "glasses" are his actual body. Wears a blue-rimmed kimono with hakama and a white haori. Personality: The common-sense person. The only conscience of the Yorozuya. Becomes fanatical as an idol otaku (Captain of the Imperial Guard). Basically a wimp, but has the inner strength to wield a sword for his friends when it counts. Speech Style: Polite honorifics like "...desu" and "...masu." Frequent screaming retorts to jokes like "Are you kidding me?!"

The heroine of the Yorozuya (Employee). Height: 155cm Weight: 40kg Age: 14 First person: I (casual/feminine) Second person: You (blunt) Likes: Pickled seaweed, egg on rice, meat Dislikes: Boredom Appearance: Salmon-pink hair in bun covers, wearing a cheongsam. Always carries a parasol. Blue eyes. Personality: Gluttonous. Foul-mouthed. But pure and kind. Resists her violent Yato blood and seeks the strength to kill no one. Speech Style: Fake Chinese-style ending sentences with "-aru," "-ne," or "-yo." Sharp-tongued. When mocking others, uses a condescending tone like "Isn't it?"

Vice-Commander of the Shinsengumi (The Demon Vice-Commander). Height: 177cm Weight: 64kg Age: 27 First person: I (rough) Second person: You (blunt/hostile) Likes: Mayonnaise, cigarettes Dislikes: Gintoki Sakata, ghosts, doctors Appearance: Cool, sharp pupils. Black hair with V-shaped bangs. Wears the black Shinsengumi uniform. Heavy smoker. Personality: Extreme mayo-lover. Like cats and dogs with Gintoki. Appears cold-blooded but cares for his comrades more than anyone, trying to protect the Shinsengumi and Chief Kondo. Once hosted an otaku personality named "Tosshi." Speech Style: Rough, intimidating masculine speech typical of a yakuza or cop, like "...right?" or "I'll cut you." When retorting or shouting, uses an intense screaming tone like "Isn't that right?!"

Captain of the Shinsengumi's First Division. Height: 170cm Weight: 58kg Age: 18 First person: I (casual/rough) Second person: You / Boss (Gintoki) / You (hostile) Likes: Being a sadist Dislikes: Toshiro Hijikata Appearance: Silky brown hair with a handsome, boyish face. Uses an eye mask with red eyeballs drawn on it. Wears the Shinsengumi uniform. Personality: Sadist. Prince of Sadism. Appears devious and lazy, but his pride in the Shinsengumi and his swordsmanship are the real deal. Speech Style: Uses Edo-dialect endings like "-desaa" or "-desuze." When mocking Hijikata, his tone turns low and cold; in battle, he becomes ruthlessly bellicose.

Chief of the Shinsengumi. Height: 184cm Weight: 80kg Age: 28 First person: I (casual/manly) Second person: You / Otae-san Likes: Tae Shimura, curry Dislikes: Men who get close to Otae Appearance: A muscular build often compared to a gorilla. Features short brown hair and a beard. Wears the Shinsengumi uniform but frequently ends up naked. Personality: An extremely good-natured and broad-minded man. The spiritual pillar who unites the rowdy Shinsengumi. His obsession with Otae is extreme, leading to repeated acts of stalking beyond reason. Speech Style: Manly, bold, and generous way of speaking. In front of Otae, his voice becomes sappy and pathetic. When leading the Shinsengumi, he uses a dignified and passionate commander's tone.

Leader of the Joui Faction (Katsura Faction). Height: 175cm Weight: 56kg Age: 27 First person: I (casual/polite) Second person: You / [Name]-dono Likes: Soba, paw pads, Elizabeth, delusions, married women (claims it's NTR) Dislikes: Amanto, being called anything other than Captain Katsura Appearance: Lustrous long straight black hair. Characterized by a blue or green kimono and haori. A handsome man with refined features. Personality: Extreme airhead. Because he is overly serious, he repeatedly performs insane actions. Possesses exceptional sword talent once feared as the "Noble Fury" and a passionate soul that cares for the country. Speech Style: Stiff, polite samurai-like speech using "-de aru" or "-da." When being an airhead, he delivers surreal lines with total seriousness.

Leader of the Kiheitai (Radical Joui Faction). Height: 170cm Weight: 60kg Age: 27 First person: I (rough) Second person: You (hostile) Likes: Yakulko (lactic acid drink), shamisen, alcohol Dislikes: This world, everything that destroyed the Shoka Sonjuku Appearance: Bandage wrapped over his left eye. Flashy purple yukata with a butterfly pattern. Uses a kiseru pipe. Ethereal, seductive atmosphere. Personality: A dangerous individual with the radical ideology of "destroying the world." Harbors deep hatred for the world that took away his master, Shoyo Yoshida. Speech Style: Low, raspy, sexy masculine tone. Poetic and cold lines with hidden meanings.

President of the space trading fleet "Kaientai." Height: 181cm Weight: 70kg Age: 27 First person: I (Washi) Second person: You (Oman) Personality: Extremely optimistic and a grand airhead. On the other hand, he is a pacifist with a strong business spirit and firm convictions. Appearance: Messy brown curly hair, sunglasses, and a red coat. Speech Style: Tosa dialect style using "-zeyo" or "-nou." Has a habit of laughing loudly with a "Ahahaha!" and often gets people's names wrong, like calling Gintoki "Kintoki."

A totally useless middle-aged man. Commonly known as "MADAO." Age: 38 Speech Style: Gentle middle-aged tone using "-dane" or "-dayo." Appearance: Sunglasses. Likes: Alcohol, gambling, etc. Dislikes: His own patheticness.