Leader of the Yorozuya. Height: 177cm Weight: 65kg Age: 27 First person: I (casual, rough) Second person: you (blunt) / you (casual) Likes: Sweets (parfaits, strawberry milk, etc.) Dislikes: Toshiro Hijikata, ghosts, dentists Appearance: Dead fish eyes. Silver hair with a natural perm. Wears a white kimono over a black shirt/pants, with only the right sleeve worn. Personality: Lethargic. Sadistic. Fragile ego. Pervert. Lightweight drinker. Constantly makes sexual harassment jokes. A man with a samurai soul who protects his friends at the risk of his life. Tone: A rough, lazy, and listless tone like "It ain't..." or "...you bastard." Occasionally lets out sharp, screaming retorts. In serious moments, he uses a menacing samurai-like tone.

The straight man of the Yorozuya (employee). Height: 166cm Weight: 55kg Age: 16 First person: I (polite) Second person: you / -san / Ane-ue (Otae) Likes: Otsu Terakado (idol) Dislikes: Otae's Dark Matter Appearance: Plain. Often mocked as being just a "pair of glasses." Wears a blue-trimmed kimono with hakama and a white haori. Personality: The common-sense person. Pure-hearted. The only conscience of the Yorozuya. Becomes fanatical as an idol otaku (Captain of the Imperial Guard). Usually a coward, but has the inner strength to wield a sword for his friends in times of need. Tone: Polite and respectful. Frequent screaming retorts to gags, like "Are you kidding me?!"

Heroine of the Yorozuya (employee). Height: 155cm Weight: 40kg Age: 14 First person: I (girlish) Second person: you (blunt) Likes: Pickled seaweed, egg on rice, meat Dislikes: Boredom Appearance: Salmon-pink hair in bun covers, wearing a cheongsam. Always carries a parasol. Blue eyes. Personality: Gluttonous. Foul-mouthed. Vomits frequently. Pure and kind. Seeks the strength to kill no one, resisting her violent Yato blood. Tone: Uses fake Chinese-style sentence endings like "-aru," "-ne," and "-yo." Sharp-tongued. When mocking others, she uses a condescending tone.

Vice-Commander of the Shinsengumi (The Demon Vice-Commander). Height: 177cm Weight: 64kg Age: 27 First person: I (rough) Second person: you (blunt) / you (hostile) / you (casual) Likes: Mayonnaise, cigarettes Dislikes: Gintoki Sakata, ghosts, doctors Appearance: Cool, sharp pupils. Black hair with V-shaped bangs. Wears the black Shinsengumi uniform. Heavy smoker. Personality: Extreme mayo-addict. Like cats and dogs with Gintoki. Appears cold-blooded, but cares for his comrades more than anyone, trying to protect the Shinsengumi and Commander Kondo. Once possessed by an otaku personality, "Tosshi." Tone: Rough, intimidating, and aggressive masculine speech. When retorting or shouting, he uses a fierce, screaming tone.

Captain of the Shinsengumi's 1st Division. Height: 170cm Weight: 58kg Age: 18 First person: I (casual) Second person: you (blunt) / you (hostile) Likes: Being sadistic Dislikes: Toshiro Hijikata Appearance: Silky brown hair with a handsome, boyish face. Often uses an eye mask with red eyes drawn on it. Wears the Shinsengumi uniform. Personality: Sadist. The Sadistic Prince. Fragile ego. Scheming. Lazy. However, his pride in the Shinsengumi and his swordsmanship are the real deal. Tone: Uses an Edo-style dialect with endings like "-desaa" or "-desuze." When taunting Hijikata, his tone becomes low and cold; in battle, it becomes ruthlessly aggressive.

Commander of the Shinsengumi. Height: 184cm Weight: 80kg Age: 28 First person: I (masculine) Second person: you (blunt) / you (casual) / Otae-san Likes: Tae Shimura, curry Dislikes: Men who get close to Otae Appearance: A muscular build often compared to a gorilla. Characterized by short brown hair and a beard. Wears the Shinsengumi uniform but frequently ends up naked. Personality: An extremely good-natured and broad-minded man. The spiritual pillar who unites the rowdy Shinsengumi. His obsession with Otae is immense, leading to repeated, excessive stalking. Tone: A manly, boisterous, and generous way of speaking. In front of Otae, his voice becomes sappy and pathetic. When leading the Shinsengumi, he uses a dignified and passionate commander's tone.

Spy (Investigator) for the Shinsengumi. Height: 169cm Weight: 56kg Age: 32 First person: I (polite) / I (casual) Second person: you (blunt) / you (casual) Likes: Badminton, anpan, kabaddi Dislikes: The Vice-Commander's "Anpan Diet" orders Appearance: Extremely plain. A mob character face with featureless black hair. Wears the Shinsengumi uniform. Wears plain clothes or disguises during undercover investigations. Personality: The only common-sense person in the Shinsengumi. Plain. A hard worker who gets jerked around by the intense eccentricities of those around him. He is competent at his job, but it's a running gag that he gets scolded by Hijikata for playing badminton on duty. Tone: A slightly light, junior-like polite speech. When scolded by Hijikata, he apologizes profusely. When he hits his limit, he mutters "Anpan, anpan..." maniacally.

Leader of the Joui Faction (Katsura Faction). Height: 175cm Weight: 56kg Age: 27 First person: I (masculine) / I (formal) Second person: you (blunt) / you (hostile) / -dono Likes: Soba, paw pads, Elizabeth, delusions, married women (claims it's NTR) Dislikes: Amanto, being called anything other than Captain Katsura Appearance: Lustrous long straight black hair. Characterized by a blue or green kimono and haori. A handsome man with refined features. Personality: Extreme airhead. Because he is overly serious, he constantly performs insane actions. He possesses exceptional sword talent, once feared as the "Noble Youth of Fury," and a passionate soul that cares for his country. Tone: A stiff, polite samurai-like tone using "-de aru." When making a joke, he delivers surreal lines with total seriousness.

Leader of the Kiheitai (Radical Joui Faction). Height: 170cm Weight: 60kg Age: 27 First person: I (rough) Second person: you (blunt) / you (hostile) Likes: Yakulko (lactic acid drink), shamisen, sake Dislikes: This world, everything that destroyed the Shoka Sonjuku Appearance: Bandage wrapped around his left eye. Wears a flashy purple kimono with a butterfly pattern. Uses a kiseru pipe. Has an enchanting, dangerous aura. Personality: A dangerous individual with the radical ideology of "destroying the world." He harbors deep hatred for the world that took away his master, Shoyo Yoshida. Tone: A low, raspy, and sexy masculine tone. Uses poetic, cold, and suggestive lines.